anti-SHIP1 antibody [SHIP-01]
![anti-SHIP1 antibody [SHIP-01]](/upload/image/products/SHIP-01_1_210_205.jpg)
![anti-SHIP1 antibody [SHIP-01]](/upload/image/products/SHIP-01_1.jpg)
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- 靶点名称:
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CAT.NO. : ARG63092
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody [SHIP-01] recognizes SHIP1 |
|---|---|
| 反应物种 | Hu |
| 应用 | FACS, WB |
| 特异性 | The clone SHIP-01 reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells. Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa. |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | SHIP-01 |
| 同位型 | IgG2a |
| 靶点名称 | SHIP1 |
| 抗原物种 | Human |
| 抗原 | Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1. |
| 偶联标记 | Un-conjugated |
| 別名 | EC 3.1.3.86; Inositol polyphosphate-5-phosphatase of 145 kDa; hp51CN; p150Ship; SHIP1; SIP-145; SHIP-1; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol phosphatase 1; Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; SHIP |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Boil for 6 min. Application note: Reducing condition. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | FACS: Human blood leukocytes WB: Ramos |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from ascites by protein-A affinity chromatography. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) and 15 mM Sodium azide |
| 抗菌剂 | 15 mM Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q92835 Human Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 |
|---|---|
| 基因名称 | INPP5D |
| 全名 | inositol polyphosphate-5-phosphatase, 145kDa |
| 背景介绍 | SHIP-1 (SH2 domain containing inositol phosphatase-1) is a 5´inositol phosphatase that regulates cell responses in lymphocytes and myeloid cells by hydrolyzing the second messenger PI(3,4,5) trisphosphate. SHIP-1 is recruited upon engagement of both inhibitory and activatory receptors, such as FcgammaRIIB, Fcgamma RIII, FcepsilonRI or cytokine and growth factor receptors, and supresses PI3K-dependent signaling, down-regulates cell migration and invasion of transformed cells and phagocytosis. SHIP-1 also serves as a scaffold for the recruitment of other proteins to the plasma membrane. |
| 生物功能 | Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. [UniProt] |
| 研究领域 | Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 133 kDa |
| 翻译后修饰 | Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding. |
检测图片 (1)
ARG63092 anti-SHIP1 antibody [SHIP-01] WB image
Western blot: THP-1 cell lysate stained with 1) Isotype Mouse IgG1 control antibody, or both 2) and 3) ARG63092 anti-SHIP1 antibody [SHIP-01].
参考文献
克隆号文献
Inactivation of SHIP1 in T-cell acute lymphoblastic leukemia due to mutation and extensive alternative splicing.
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