anti-SIRT3 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG63826
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Product Details
概述
| 产品描述 | Goat Polyclonal antibody recognizes SIRT3 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 特异性 | This antibody is expected to recognize both reported isoforms (NP_036371.1 and NP_001017524.1). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SIRT3 |
| 抗原物种 | Human |
| 抗原 | C-QRETGKLDGPDK |
| 偶联标记 | Un-conjugated |
| 別名 | NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2L3; hSIRT3; EC 3.5.1.-; Regulatory protein SIR2 homolog 3; SIR2-like protein 3 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q9NTG7 Human NAD-dependent protein deacetylase sirtuin-3, mitochondrial |
|---|---|
| 背景介绍 | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq, Jul 2008] |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody |
| 预测分子量 | 44 kDa |
| 翻译后修饰 | Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity. |
检测图片 (2)
ARG63826 anti-SIRT3 antibody WB image
Western Blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG63826 anti-SIRT3 antibody at 0.2 µg/ml dilution.
ARG63826 anti-SIRT3 antibody WB image
Western Blot: 1). Mock transfection; 2) SIRT3 (RC200190) expressing plasmid transfected HEK293 cell lysate standed with ARG63826 anti-SIRT3 antibody
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