anti-SIRT4 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes SIRT4
  • 反应物种: Hu
  • 预测物种: Ms, Rat, Cow, Dog, Pig
  • 应用: IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: SIRT4
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63423
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes SIRT4
反应物种Hu
预测物种Ms, Rat, Cow, Dog, Pig
应用IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称SIRT4
抗原物种Human
抗原SRCGELLPLIDPC
偶联标记Un-conjugated
別名SIR2L4; NAD-dependent ADP-ribosyltransferase sirtuin-4; NAD-dependent protein deacetylase sirtuin-4; SIR2-like protein 4; EC 3.5.1.-; NAD-dependent protein lipoamidase sirtuin-4, mitochondrial; EC 2.4.2.-; Regulatory protein SIR2 homolog 4
应用说明
应用建议
应用推荐稀释比
IHC-P2 - 3 µg/ml
WB1 - 3 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 23409 Human SIRT4

Swiss-port # Q9Y6E7 Human NAD-dependent protein lipoamidase sirtuin-4, mitochondrial

背景介绍This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]
研究领域Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量35 kDa
检测图片 (4)
  • ARG63423 anti-SIRT4 antibody WB image

    Western Blot: 1). Mock transfection; 2) SIRT4 (RC212226) expressing plasmid transfected HEK293 cell lysate standed with ARG63423 anti-SIRT4 antibody.

  • ARG63423 anti-SIRT4 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human skeletal muscle tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63423 anti-SIRT4 antibody at 2.5 µg/ml dilution followed by AP-staining.

  • ARG63423 anti-SIRT4 antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Pancreas. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63423 anti-SIRT4 antibody at 2 µg/ml dilution followed by AP-staining.

  • ARG63423 anti-SIRT4 antibody WB image

    Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63423 anti-SIRT4 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.

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