anti-Smac / Diablo antibody [19F2]

CAT.NO. : ARG57123

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [19F2] recognizes Smac / Diablo
反应物种	Hu
应用	FACS, ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	19F2
同位型	IgG2a, kappa
靶点名称	Smac / Diablo
抗原物种	Human
抗原	Recombinant fragment around aa. 56-239 of Human Smac / Diablo
偶联标记	Un-conjugated
別名	Smac; Second mitochondria-derived activator of caspase; Diablo homolog, mitochondrial; SMAC; Direct IAP-binding protein with low pI; DFNA64

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein A.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 56616 Human DIABLO Swiss-port # Q9NR28 Human Diablo homolog, mitochondrial
基因名称	DIABLO
全名	diablo, IAP-binding mitochondrial protein
背景介绍	This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Overexpression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
生物功能	Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. [UniProt]
预测分子量	27 kDa
翻译后修饰	Ubiquitinated by BIRC7/livin.

检测图片 (4)

ARG57123 anti-Smac / Diablo antibody [19F2] ICC/IF image
Immunofluorescence: HeLa cells line stained with ARG57123 anti-Smac / Diablo antibody [19F2] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG57123 anti-Smac / Diablo antibody [19F2] FACS image
Flow Cytometry: HeLa cell line stained with ARG57123 anti-Smac / Diablo antibody [19F2] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
ARG57123 anti-Smac / Diablo antibody [19F2] WB image
Western blot: 40 µg of Jurkat cell lysate stained with ARG57123 anti-Smac / Diablo antibody [19F2] at 1:3000.
ARG57123 anti-Smac / Diablo antibody [19F2] WB image
Western blot: 40 µg of 1) 293T, 2) A431, 3) Hep3B, and 4) MCF7 cell lysates stained with ARG57123 anti-Smac / Diablo antibody [19F2] at 1:1000.

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