anti-Smad 2 phospho (Thr220) antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes Smad 2 phospho (Thr220)
  • 反应物种: Hu, Ms, Rat
  • 应用: WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Smad 2
  • 抗原物种: Human
  • 抗原: Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2.
  • Brand:
CAT.NO. : ARG51796
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes Smad 2 phospho (Thr220)
反应物种Hu, Ms, Rat
应用WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称Smad 2
抗原物种Human
抗原Peptide sequence around phosphorylation site of threonine 220 (P-E-T(p)-P-P) derived from Human Smad2.
偶联标记Un-conjugated
別名SMAD family member 2; MADR2; MADH2; Mothers against decapentaplegic homolog 2; JV18; Smad2; hMAD-2; Mad-related protein 2; hSMAD2; Mothers against DPP homolog 2; JV18-1; MAD homolog 2; SMAD 2
应用说明
应用建议
应用推荐稀释比
WB1:500 - 1:1000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
缓冲液PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 17126 Mouse SMAD2

GeneID: 29357 Rat SMAD2

GeneID: 4087 Human SMAD2

基因名称SMAD2
全名SMAD family member 2
背景介绍Transcriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
生物功能Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]
研究领域Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
预测分子量52 kDa
翻译后修饰Phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases. TGF-beta-induced Ser-465/467 phosphorylation declines progressively in a KMT5A-dependent manner. Able to interact with SMURF2 when phosphorylated on Ser-465/467, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, phosphorylated on Ser-240 by CaMK2. Phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. Phosphorylated by PDPK1.
In response to TGF-beta, ubiquitinated by NEDD4L; which promotes its degradation. Monoubiquitinated, leading to prevent DNA-binding (By similarity). Deubiquitination by USP15 alleviates inhibition and promotes activation of TGF-beta target genes (PubMed:21947082). Ubiquitinated by RNF111, leading to its degradation: only SMAD2 proteins that are 'in use' are targeted by RNF111, RNF111 playing a key role in activating SMAD2 and regulating its turnover (By similarity).
Acetylated on Lys-19 by coactivators in response to TGF-beta signaling, which increases transcriptional activity. Isoform short: Acetylation increases DNA binding activity in vitro and enhances its association with target promoters in vivo. Acetylation in the nucleus by EP300 is enhanced by TGF-beta.
检测图片 (1)
  • ARG51796 anti-Smad 2 phospho (Thr220) antibody WB image

    Western blot: Extracts from HeLa cells untreated or treated with PMA stained with ARG51796 anti-Smad 2 phospho (Thr220) antibody.

参考文献

Kangquan Recipe Regulates the Expression of BAMBI Protein via the TGF-β/Smad Signaling Pathway to Inhibit Benign Prostatic Hyperplasia in Rats.

WB / Rat

Chen Wenfan et al.
Evid Based Complement Alternat Med.,  (2019)

publication_link

 

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The ATRQβ-001 vaccine improves cardiac function and prevents postinfarction cardiac remodeling in mice.

WB / Mouse

Pan Yajie et al.
Hypertens Res.,  (2018)

publication_link

 

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