anti-SMAD9 antibody
CAT.NO. : ARG63942
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概述
产品描述 | Goat Polyclonal antibody recognizes SMAD9 |
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反应物种 | Hu |
预测物种 | Ms, Rat, Dog |
应用 | IHC-P, WB |
特异性 | This antibody is not expected to cross-react with SMAD1 and SMAD5. |
宿主 | Goat |
克隆 | Polyclonal |
同位型 | IgG |
靶点名称 | SMAD9 |
抗原物种 | Human |
抗原 | C-HSEPLMPHNATYPD |
偶联标记 | Un-conjugated |
別名 | Mothers against decapentaplegic homolog 9; MADH9; SMAD8A; Smad9; SMAD8B; Mothers against DPP homolog 9; MADH6; PPH2; Madh6; SMAD family member 9; SMAD 9; MAD homolog 9; SMAD8 |
应用说明
应用建议 |
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应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
形式 | Liquid |
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纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
抗菌剂 | 0.02% Sodium azide |
稳定剂 | 0.5% BSA |
浓度 | 0.5 mg/ml |
存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
数据库连接 | Swiss-port # O15198 Human Mothers against decapentaplegic homolog 9 |
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背景介绍 | The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010] |
研究领域 | Cancer antibody; Developmental Biology antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody |
预测分子量 | 52 kDa |
翻译后修饰 | Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. |
检测图片 (2)
ARG63942 anti-SMAD9 antibody WB image
Western Blot: Human Skin lysate (35 µg protein in RIPA buffer) stained with ARG63942 anti-SMAD9 antibody at 0.3 µg/ml dilution.
ARG63942 anti-SMAD9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63942 anti-SMAD9 antibody at 5 µg/ml dilution followed by AP-staining.
