anti-SNAP25 antibody [4E11]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [4E11] recognizes SNAP25
  • 反应物种: Hu, Ms
  • 应用: ICC/IF, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 4E11
  • 同位型: IgG1, kappa
  • 靶点名称: SNAP25
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG56942
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Product Details
概述
产品描述Mouse Monoclonal antibody [4E11] recognizes SNAP25
反应物种Hu, Ms
应用ICC/IF, WB
宿主Mouse
克隆Monoclonal
克隆号4E11
同位型IgG1, kappa
靶点名称SNAP25
抗原物种Human
抗原Recombinant fragment around aa. 1-206 of Human SNAP25.
偶联标记Un-conjugated
別名Super protein; Synaptosomal-associated 25 kDa protein; bA416N4.2; RIC4; SUP; dJ1068F16.2; SNAP; RIC-4; CMS18; SEC9; SNAP-25; Synaptosomal-associated protein 25
应用说明
应用建议
应用推荐稀释比
ICC/IFAssay-dependent
WBAssay-dependent
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂10% Glycerol
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 20614 Mouse SNAP25

GeneID: 6616 Human SNAP25

Swiss-port # P60879 Mouse Synaptosomal-associated protein 25

Swiss-port # P60880 Human Synaptosomal-associated protein 25

基因名称SNAP25
全名synaptosomal-associated protein, 25kDa
背景介绍Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
生物功能t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells. [UniProt]
预测分子量23 kDa
翻译后修饰Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association (By similarity).
检测图片 (4)
  • ARG56942 anti-SNAP25 antibody [4E11] ICC/IF image

    Immunoflorescense: U87MG cell line stained with ARG56942 anti-SNAP25 antibody [4E11] at 1:100 (Green).

    DAPI (Blue) for nucleus staining.

  • ARG56942 anti-SNAP25 antibody [4E11] FACS image

    Flow Cytometry: U87MG cell line stained with ARG56942 anti-SNAP25 antibody [4E11] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was Mouse IgG (black line).

  • ARG56942 anti-SNAP25 antibody [4E11] WB image

    Western blot: 30 µg of Mouse brain stained with ARG56942 anti-SNAP25 antibody [4E11] at 1:2000.

  • ARG56942 anti-SNAP25 antibody [4E11] WB image

    Western blot: 10 µg of 1) 293T cell lysate, 2) SNAP25 Transfected 293T cell lysate stained with ARG56942 anti-SNAP25 antibody [4E11] at 1:3000.

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