anti-SOD1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes SOD1 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Dog, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SOD1 |
| 抗原物种 | Human |
| 抗原 | C-SRKHGGPKDEERH |
| 偶联标记 | Un-conjugated |
| 別名 | homodimer; EC 1.15.1.1; SOD; HEL-S-44; Superoxide dismutase [Cu-Zn]; ALS1; Superoxide dismutase 1; IPOA; ALS; hSod1 |
| 应用建议 |
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|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
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| 基因名称 | SOD1 |
| 全名 | superoxide dismutase 1, soluble |
| 背景介绍 | The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008] |
| 产品亮点 | Related Antibody Duos and Panels: ARG30274 SOD1 and SOD2 Antibody Duo Related products: SOD1 antibodies; SOD1 ELISA Kits; SOD1 Duos / Panels; Anti-Goat IgG secondary antibodies; Related poster download: The Structure & Functions of Mitochondria.pdf |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody |
| 预测分子量 | 16 kDa |
| 翻译后修饰 | Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. Palmitoylation helps nuclear targeting and decreases catalytic activity. Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity. |
ARG64241 anti-SOD1 antibody WB image
Western blot: 30 µg of HeLa cell lysate stained with ARG64241 anti-SOD1 antibody at 1:2000 dilution.
ARG64241 anti-SOD1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed U2OS cells, permeabilized with 0.15% Triton. Cells were stained with ARG64241 anti-SOD1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized Goat IgG (green) at 10 µg/ml dilution.
ARG64241 anti-SOD1 antibody WB image
Western blot: 30 µg of Mouse brain lysate stained with ARG64241 anti-SOD1 antibody at 1:2000 dilution.
ARG64241 anti-SOD1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed A431 cells, permeabilized with 0.15% Triton. Cells were stained with ARG64241 anti-SOD1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized Goat IgG (green) at 10 µg/ml dilution.
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