anti-SPRY1 / Sprouty 1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
-
Brand:
Product Details
| 产品描述 | Goat Polyclonal antibody recognizes SPRY1 / Sprouty 1 |
|---|---|
| 反应物种 | Hu, Ms |
| 应用 | FACS, ICC/IF, IHC-P, WB |
| 特异性 | NP_005832.1 and NP_955359.1 represent varients of the same protein. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SPRY1 / Sprouty 1 |
| 抗原物种 | Human |
| 抗原 | CPSRGQGKPS |
| 偶联标记 | Un-conjugated |
| 別名 | hSPRY1; Protein sprouty homolog 1; Spry-1 |
| 应用建议 |
| ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 基因名称 | SPRY1 |
| 全名 | sprouty homolog 1, antagonist of FGF signaling (Drosophila) |
| 生物功能 | May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis. [UniProt] |
| 研究领域 | Signaling Transduction antibody |
| 预测分子量 | 35 kDa |
ARG63617 anti-SPRY1 / Sprouty 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HeLa cells permeabilized with 0.15% Triton. Cells were stained with ARG63617 anti-SPRY1 / Sprouty 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining.
ARG63617 anti-SPRY1 / Sprouty 1 antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63617 anti-SPRY1 / Sprouty 1 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
ARG63617 anti-SPRY1 / Sprouty 1 antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63617 anti-SPRY1 / Sprouty 1 antibody at 10 µg/ml dilution for 1 hour (blue line) or Goat IgG for isotype control (black line), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
ARG63617 anti-SPRY1 / Sprouty 1 antibody ICC/IF image
Immunofluorescence: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63617 anti-SPRY1 / Sprouty 1 antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining.
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