anti-SSP29 / ANP32B antibody
CAT.NO. : ARG63289
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概述
| 产品描述 | Goat Polyclonal antibody recognizes SSP29 / ANP32B |
|---|---|
| 反应物种 | Hu, Ms, Rat, Pig |
| 预测物种 | Cow, Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125). |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | SSP29 / ANP32B |
| 抗原物种 | Human |
| 抗原 | C-KRKRETDDEGEDD |
| 偶联标记 | Un-conjugated |
| 別名 | Silver-stainable protein SSP29; PHAPI2; APRIL; SSP29; Acidic protein rich in leucines; Acidic leucine-rich nuclear phosphoprotein 32 family member B; Putative HLA-DR-associated protein I-2 |
应用说明
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ANP32B |
| 全名 | acidic (leucine-rich) nuclear phosphoprotein 32 family, member B |
| 生物功能 | Multifunctional protein working as a cell cycle progression factor as well as a cell survival factor. Required for the progression from the G1 to the S phase. Anti-apoptotic protein which functions as a caspase-3 inhibitor. Has no phosphatase 2A (PP2A) inhibitor activity (By similarity). Exhibits histone chaperone properties, stimulating core histones to assemble into a nucleosome. [UniProt] |
| 研究领域 | Cell Biology and Cellular Response antibody; Cell Death antibody |
| 预测分子量 | 29 kDa |
| 翻译后修饰 | Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity). |
检测图片 (3)
ARG63289 anti-SSP29 / ANP32B antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Breast. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63289 anti-SSP29 / ANP32B antibody at 3.8 µg/ml dilution followed by AP-staining.
ARG63289 anti-SSP29 / ANP32B antibody WB image
Western blot: 35 µg of Human tonsil (A), Rat spleen (B) and Pig spleen (C) lysates (in RIPA buffer) stained with ARG63289 anti-SSP29 / ANP32B antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
ARG63289 anti-SSP29 / ANP32B antibody WB image
Western blot: 35 µg of NIH/3T3 cell lysate (in RIPA buffer) stained with ARG63289 anti-SSP29 / ANP32B antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
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