anti-STAP2 / BKS antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes STAP2 / BKS
  • 反应物种: Hu
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: STAP2 / BKS
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG63646
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes STAP2 / BKS
反应物种Hu
应用IHC-P, WB
特异性This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称STAP2 / BKS
抗原物种Human
抗原C-ELQKKLEKRRALEH
偶联标记Un-conjugated
別名Signal-transducing adaptor protein 2; BRK substrate; STAP-2; Breast tumor kinase substrate; BKS
应用说明
应用建议
应用推荐稀释比
IHC-PAssay - dependent
WB0.5 - 1.5 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 55620 Human STAP2

Swiss-port # Q9UGK3 Human Signal-transducing adaptor protein 2

背景介绍This gene encodes the substrate of breast tumor kinase, an Src-type non-receptor tyrosine kinase. The encoded protein possesses domains and several tyrosine phosphorylation sites characteristic of adaptor proteins that mediate the interactions linking proteins involved in signal transduction pathways. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
研究领域Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
预测分子量45 kDa
翻译后修饰Phosphorylated on tyrosine. Tyr-250 may be important for interaction with kinases. Phosphorylated by PTK6 at Tyr-250 modulates PTK6-mediated STAT3 activation. Tyr-22 and Tyr-322 appears to be phosphorylated by SRC.
检测图片 (4)
  • ARG63646 anti-STAP2 / BKS antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human small intestine tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63646 anti-STAP2 / BKS antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG63646 anti-STAP2 / BKS antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Cortex. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63646 anti-STAP2 / BKS antibody at 3.8 µg/ml dilution followed by AP-staining.

  • ARG63646 anti-STAP2 / BKS antibody WB image

    Western blot: 35 µg of Human heart lysate (in RIPA buffer) stained with ARG63646 anti-STAP2 / BKS antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.

  • ARG63646 anti-STAP2 / BKS antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63646 anti-STAP2 / BKS antibody at 3.75 µg/ml dilution followed by AP-staining.

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