anti-Sulfatase 2 antibody [2B4]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [2B4] recognizes Sulfatase 2.This product recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Studies have linked Sulf-2 with several cancers, including breast, hepatocellular, and pancreatic cancer, and recently with non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010). Mature human Sulf-2 is a heterodimer consisting of a 50kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75kDa N-terminal subunit which contains the catalytic centre. Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.
  • 反应物种: Hu, Ms
  • 应用: ELISA, IHC-P, WB
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: 2B4
  • 同位型: IgG1
  • 靶点名称: Sulfatase 2
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG23581
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Product Details
概述
产品描述Mouse Monoclonal antibody [2B4] recognizes Sulfatase 2.
This product recognizes an epitope within the C-terminal (CT) subunit of Sulfatase 2 (Sulf-2). Sulf-2 is a novel extracellular heparan sulfate 6-O-endosulfatase, which selectively removes the critical sulfate group from the polysaccharide side chain of heparan sulfate proteoglycans, major components of the extracellular matrix, involved in cell differentiation, proliferation and migration. Sulf-2 acts as a modulator of several signalling proteins including Wnt proteins, bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs), and is a direct transcriptional target of p53. Studies have linked Sulf-2 with several cancers, including breast, hepatocellular, and pancreatic cancer, and recently with non-small-cell lung carcinomas (Lemjabbar-Alaoui et al. 2010). Mature human Sulf-2 is a heterodimer consisting of a 50kDa C-terminal subunit, essential for both glucosamine 6-O-sulfate-endosulfatase and arylsulfatase activity, and a 75kDa N-terminal subunit which contains the catalytic centre. Mouse anti Human Sulfatase 2 (C-Terminal) antibody, clone 2B4recognizes Sulfatase 2, showing no cross-reactivity with Sulfatase 1 in either human or mouse.
反应物种Hu, Ms
应用ELISA, IHC-P, WB
宿主Mouse
克隆Monoclonal
克隆号2B4
同位型IgG1
靶点名称Sulfatase 2
抗原物种Human
抗原Recombinant Human Sulfatase 2.
偶联标记Un-conjugated
別名HSULF-2; hSulf-2; Extracellular sulfatase Sulf-2; EC 3.1.6.-
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
应用说明IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0).
WB: This product detects a major band of ~ 50 kDa in Sulf-2 transfected HEK293 cell lysates. A band at 135 kDa (unprocessed protein), and a fragment at 37 kDa, may also be present.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS and 0.09% Sodium azide.
抗菌剂0.09% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 55959 Human SULF2

GeneID: 72043 Mouse SULF2

Swiss-port # Q8CFG0 Mouse Extracellular sulfatase Sulf-2

Swiss-port # Q8IWU5 Human Extracellular sulfatase Sulf-2

基因名称SULF2
全名sulfatase 2
背景介绍Heparan sulfate proteoglycans (HSPGs) act as coreceptors for numerous heparin-binding growth factors and cytokines and are involved in cell signaling. Heparan sulfate 6-O-endosulfatases, such as SULF2, selectively remove 6-O-sulfate groups from heparan sulfate. This activity modulates the effects of heparan sulfate by altering binding sites for signaling molecules (Dai et al., 2005 [PubMed 16192265]).[supplied by OMIM, Mar 2008]
生物功能Exhibits arylsulfatase activity and highly specific endoglucosamine-6-sulfatase activity. It can remove sulfate from the C-6 position of glucosamine within specific subregions of intact heparin. [UniProt]
预测分子量100 kDa
翻译后修饰The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. [UniProt]
检测图片 (1)
  • ARG23581 anti-Sulfatase 2 antibody [2B4] IHC-P image

    Immunohistochemistry: Paraffin-embedded HEK293 cells transfected with SULF2 stained with ARG23581 anti-Sulfatase 2 antibody [2B4].

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