anti-TAF15 antibody [4D71]

CAT.NO. : ARG10751

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Size:

50 μl

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

概述

产品描述	Mouse Monoclonal antibody [4D71] recognizes TAF15
反应物种	Hu, Ms
应用	ICC/IF, IHC-Fr, WB
宿主	Mouse
克隆	Monoclonal
克隆号	4D71
同位型	IgG1
靶点名称	TAF15
抗原物种	Human
抗原	Full length recombinant Human TAF15 expressed in and purified from E. coli.
偶联标记	Un-conjugated
別名	RBP56; TATA-binding protein-associated factor 2N; RNA-binding protein 56; TAF2N; II; Npl3; TAF; TAFII68; 68 kDa TATA-binding protein-associated factor

应用说明

应用建议

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Affinity purification.
缓冲液	PBS and 50% Glycerol.
稳定剂	50% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 8148 Human TAF15 Swiss-port # Q92804 Human TATA-binding protein-associated factor 2N
基因名称	TAF15
全名	TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa
背景介绍	This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
生物功能	RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II). [UniProt]
预测分子量	62 kDa
翻译后修饰	Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. ADP-ribosylated during genotoxic stress.

检测图片 (4)

ARG10751 anti-TAF15 antibody [4D71] ICC/IF image
Immunocytochemistry: HeLa cell cultures stained with ARG10751 anti-TAF15 antibody [4D71] (green) and co-stained with chicken antibody to vimentin (red). ARG10751 anti-TAF15 antibody [4D71] reveals a granular nuclear localization typical of many RNA associated proteins. Blue is a DNA stain.
ARG10751 anti-TAF15 antibody [4D71] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG10751 anti-TAF15 antibody [4D71] (red) at 1:500 dilution and costained with Rabbit pAb to GAPDH (green) at 1:1000 dilution. Hoechst (blue) for nuclear staining.
Clone 4D71 reveals TAF15 protein expressed exclusively in the nuclei of the cells, while GAPDH antibody produces strong cytoplasmic staining.
ARG10751 anti-TAF15 antibody [4D71] WB image
Western blot: 20 µg of 1) HeLa, and 2) Hek293 cell lysates stained with ARG10751 anti-TAF15 antibody [4D71] at 1: 1000 dilution.
ARG10751 anti-TAF15 antibody [4D71] WB image
Western blot: NIH/3T3 (cytosol), NIH/3T3 (nuclear), HeLa (cytosol) and HeLa (nuclear). The blots were stained with ARG10751 anti-TAF15 antibody [4D71] (green) at 1:1000 dilution.

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