anti-TAP1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG59144
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TAP1 |
|---|---|
| 反应物种 | Hu |
| 预测物种 | Ms, Rat, Cow, Gpig, Hrs, Rb, Zfsh |
| 应用 | IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TAP1 |
| 抗原物种 | Human |
| 抗原 | Synthetic peptide around the middle region of Human TAP1. (within the following region: LVTFVLYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFEYLDRTPRCPPSGL) |
| 偶联标记 | Un-conjugated |
| 別名 | Really interesting new gene 4 protein; ABC17; TAP1*0102N; ATP-binding cassette sub-family B member 2; TAP1N; RING4; Antigen peptide transporter 1; ABCB2; APT1; Peptide transporter involved in antigen processing 1; PSF-1; D6S114E; PSF1; Peptide transporter PSF1; Peptide transporter TAP1; Peptide supply factor 1 |
应用说明
| 预测反应性说明 | Predicted Homology Based On Immunogen Sequence: Cow: 92%; Guinea Pig: 86%; Horse: 93%; Mouse: 100%; Rabbit: 92%; Rat: 100%; Zebrafish: 83% | ||||||
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| 应用建议 |
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| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| 阳性对照 | MCF7 | ||||||
| 实际分子量 | ~ 70 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Affinity purified. |
| 缓冲液 | PBS, 0.09% (w/v) Sodium azide and 2% Sucrose. |
| 抗菌剂 | 0.09% (w/v) Sodium azide |
| 稳定剂 | 2% Sucrose |
| 浓度 | Batch dependent: 0.5 - 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TAP1 |
| 全名 | transporter 1, ATP-binding cassette, sub-family B (MDR/TAP) |
| 背景介绍 | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is involved in the pumping of degraded cytosolic peptides across the endoplasmic reticulum into the membrane-bound compartment where class I molecules assemble. Mutations in this gene may be associated with ankylosing spondylitis, insulin-dependent diabetes mellitus, and celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2014] |
| 生物功能 | Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. [UniProt] |
| 细胞定位 | Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=The transmembrane segments seem to form a pore in the membrane. [UniProt] |
| 预测分子量 | 87 kDa |
检测图片 (3)
ARG59144 anti-TAP1 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung stained with ARG59144 anti-TAP1 antibody (orange-red) at 1:100 dilution.
ARG59144 anti-TAP1 antibody WB image
Western blot: MCF7 cell lysate stained with ARG59144 anti-TAP1 antibody at 0.2 - 1 µg/ml dilution.
ARG59144 anti-TAP1 antibody WB image
Western blot: HeLa cell lysate stained with ARG59144 anti-TAP1 antibody at 1 µg/ml dilution.
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