anti-TPMT antibody [2E7]

CAT.NO. : ARG57762

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Size:

50 μl

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Product Details

概述

产品描述	Mouse Monoclonal antibody [2E7] recognizes TPMT
反应物种	Hu
应用	ICC/IF, WB
宿主	Mouse
克隆	Monoclonal
克隆号	2E7
同位型	IgG1, kappa
靶点名称	TPMT
抗原物种	Human
抗原	Recombinant protein around aa. 1-245 of Human TPMT.
偶联标记	Un-conjugated
別名	Thiopurine methyltransferase; Thiopurine S-methyltransferase; EC 2.1.1.67

应用说明

应用建议

应用	推荐稀释比
ICC/IF	1:100
WB	1:1000

应用说明

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

属性

形式	Liquid
纯化	Purification with Protein G.
缓冲液	PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
抗菌剂	0.02% Sodium azide
稳定剂	10% Glycerol
浓度	1 mg/ml
存放说明	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项	For laboratory research only, not for drug, diagnostic or other use.

生物信息

数据库连接	GeneID: 7172 Human TPMT Swiss-port # P51580 Human Thiopurine S-methyltransferase
基因名称	TPMT
全名	thiopurine S-methyltransferase
背景介绍	This gene encodes the enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals, causing thiopurine S-methyltransferase deficiency. Related pseudogenes have been identified on chromosomes 3, 18 and X. [provided by RefSeq, Aug 2014]
生物功能	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine. [UniProt]
预测分子量	28 kDa

检测图片 (3)

ARG57762 anti-TPMT antibody [2E7] ICC/IF image
Immunofluorescence: U87MG cells stained with ARG57762 anti-TPMT antibody [2E7] (green) at 1:100 dilution and DAPI (blue).
ARG57762 anti-TPMT antibody [2E7] WB image
Western blot: 40 µg of Hep3B cell lysate stained with ARG57762 anti-TPMT antibody [2E7] at 1:1000 dilution.
ARG57762 anti-TPMT antibody [2E7] WB image
Western blot: 50 ng of TPMT recombinant protein and 40 µg of HepG2 cell lysates stained with ARG57762 anti-TPMT antibody [2E7] at 1:1000 dilution.

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