anti-TRAF6 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原:
- 偶联标记:
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Brand:
CAT.NO. : ARG54683
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes TRAF6 |
|---|---|
| 反应物种 | Hu, Ms, Rat |
| 应用 | ELISA, ICC/IF, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | TRAF6 |
| 抗原 | Stnthetic peptide (14 aa) within the last 50 aa of Human TRAF6. |
| 偶联标记 | Un-conjugated |
| 別名 | E3 ubiquitin-protein ligase TRAF6; RNF85; EC 6.3.2.-; RING finger protein 85; Interleukin-1 signal transducer; MGC:3310; TNF receptor-associated factor 6 |
应用说明
| 应用建议 |
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|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | PC-3 Cell Lysate |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Ion exchange chromatography. |
| 缓冲液 | PBS and 0.02% Sodium azide |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TRAF6 |
| 全名 | TNF receptor-associated factor 6, E3 ubiquitin protein ligase |
| 背景介绍 | Signals from the IL-1 receptor (IL-1R)/Toll-like receptor (TLR) and TNF receptor (TNFR) superfamilies are critical for regulating the function of antigen-presenting cells. Signals transduced by these molecules lead to increased expression and activation of transcription factors such as NF-κB. TNF receptor-associated factor 6 (TRAF6) is unique in that it is a signaling adapter molecule common to both families. TRAF6 is important in cytokine production, dendritic cell (DC) maturation, and the T cell stimulatory capacity of DCs in response to TLR and CD40 ligands. It can be activated in the IL-1R/TLR signaling pathway by IL-1 receptor-associated kinase 1 (IRAK-1) or by other TLR adaptor molecules such as TRIF. Also, it has been shown that TRAF6 can interact directly with TNFR family members CD40 and RANK. |
| 生物功能 | E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. [UniProt] |
| 产品亮点 | Related products: Anti-Rabbit IgG secondary antibodies; Related poster download: The NF-kappa B Pathways.pdf Toll-like Receptor.pdf |
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Signaling Transduction antibody |
| 预测分子量 | 60 kDa |
| 翻译后修饰 | Sumoylated on Lys-124, Lys-142 and Lys-453 with SUMO1. Polyubiquitinated on Lys-124; after cell stimulation with IL-1-beta or TGF-beta. This ligand-induced cell stimulation leads to dimerization/oligomerization of TRAF6 molecules, followed by auto-ubiquitination which involves UBE2N and UBE2V1 and leads to TRAF6 activation. This 'Lys-63' site-specific poly-ubiquitination appears to be associated with the activation of signaling molecules. Endogenous autoubiquitination occurs only for the cytoplasmic form. Deubiquitinated by USP10 in a TANK-dependent manner, leading to the negative regulation of NF-kappaB signaling upon DNA damage (PubMed:25861989). |
检测图片 (3)
ARG54683 anti-TRAF6 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54683 anti-TRAF6 antibody at 1:50 dilution. Left: primary antibody (red). Right: primary antibody and DAPI (Merge).
Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
ARG54683 anti-TRAF6 antibody ICC/IF image
Immunocytochemistry: K562 cells stained with ARG54683 anti-TRAF6 antibody at 0.5 μg/ml.
ARG54683 anti-TRAF6 antibody WB image
Western blot: PC-3 cell lysates stained with ARG54683 anti-TRAF6 antibody at 1 μg/ml in the presence (A) or absence (B) of 1 μg blocking peptide.
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