anti-Transferrin antibody [HTF-14] (low endotoxin)

Key features and details

  • 产品描述: Azide free and low endotoxin Mouse Monoclonal antibody [HTF-14] recognizes Transferrin
  • 反应物种: Hu, Pig, Rb
  • 不反应物种: Cow, Dog, Hrs, Sheep
  • 应用: ELISA, FuncSt, ICC/IF, IHC-P, RIA, WB
  • 特异性: The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: HTF-14
  • 同位型: IgG1
  • Brand:
CAT.NO. : ARG65462
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Product Details
概述
产品描述Azide free and low endotoxin Mouse Monoclonal antibody [HTF-14] recognizes Transferrin
反应物种Hu, Pig, Rb
不反应物种Cow, Dog, Hrs, Sheep
应用ELISA, FuncSt, ICC/IF, IHC-P, RIA, WB
特异性The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
宿主Mouse
克隆Monoclonal
克隆号HTF-14
同位型IgG1
靶点名称Transferrin
抗原物种Pig
抗原Purified porcine transferrin.
偶联标记Un-conjugated
別名Beta-1 metal-binding globulin; Siderophilin; Transferrin; PRO1557; TFQTL1; Serotransferrin; PRO2086
应用说明
应用建议
应用推荐稀释比
ELISAAssay-dependent
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-P10 µg/ml
RIAAssay-dependent
WB1 - 2 µg/ml
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified by precipitation and chromatography.
纯化说明0.2 µm filter sterilized. Endotoxin level is less than 0.01 EU/µg of the protein.
缓冲液PBS
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 100009267 Rabbit TF

GeneID: 396996 Pig TF

GeneID: 7018 Human TF

基因名称TF
全名transferrin
背景介绍This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]
生物功能Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. [UniProt]
细胞定位Secreted. [UniProt]
预测分子量77 kDa
检测图片 (2)
  • ARG65462 anti-Transferrin antibody [HTF-14] (low endotoxin) IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG65462 anti-Transferrin antibody [HTF-14] (low endotoxin).

  • ARG65462 anti-Transferrin antibody [HTF-14] (low endotoxin) WB image

    Western blot: Human Transferrin with different lodings. 1) 5 µg, 2) 3 µg, 3) 1 µg, M) marker, 4) 1 µg, 5) 3 µg, and 6) 5 µg stained with ARG65462 anti-Transferrin antibody [HTF-14] (low endotoxin).

    Lane 1-3: Reducing condition. Lane 4-6: Non-reduring condition.

参考文献

克隆号文献

Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface.

Salvati A et al.
Nat Nanotechnol.,  (2013)

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Transferrin coated nanoparticles: study of the bionano interface in human plasma.

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Pitek AS et al.
PLoS One.,  (2012)

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Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity.

Bártek J et al.
Immunol Lett.,  (1982)

publication_link

 

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Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding.

Bártek J et al.
Br J Haematol.,  (1985)

publication_link

 

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