anti-TRIM71 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes TRIM71
  • 反应物种: Hu, Ms, Rat
  • 应用: ELISA, ICC/IF, IHC, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: TRIM71
  • 抗原: Synthetic peptide (15 aa) within aa. 680-730 of Human TRIM71.
  • 偶联标记: Un-conjugated
  • Brand:
CAT.NO. : ARG54665
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes TRIM71
反应物种Hu, Ms, Rat
应用ELISA, ICC/IF, IHC, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称TRIM71
抗原Synthetic peptide (15 aa) within aa. 680-730 of Human TRIM71.
偶联标记Un-conjugated
別名EC 6.3.2.-; Tripartite motif-containing protein 71; Protein lin-41 homolog; LIN-41; LIN41; E3 ubiquitin-protein ligase TRIM71
应用说明
应用建议
应用推荐稀释比
ELISAAssay-Dependent
ICC/IF20 μg/mL
IHC5 μg/mL
WB1 - 2 μg/mL
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Human Brain Tissue Lysate
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液PBS and 0.02% Sodium azide
抗菌剂0.02% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 131405 Human TRIM71

GeneID: 301042 Rat TRIM71

GeneID: 636931 Mouse TRIM71

基因名称TRIM71
全名tripartite motif containing 71, E3 ubiquitin protein ligase
背景介绍TRIM71 Antibody: TRIM71 is the mammalian ortholog to the C. elegans heterochronic protein Lin41, a protein that is thought to be important in postembryonic development, and is genetically and biochemically downstream of both Shh and Fgf signaling pathways. Similar to its C. elegans homolog, TRIM71 is a target of the microRNA let-7 and is likely to play a role in mammalian development. Recent experiments have indicated that TRIM71 is an E3 ubiquitin ligase and can interact with the Dicer and the Argonaute proteins Ago1, Ago2, and Ago4. Overexpression and depletion of TRIM71 led to inverse changes in Ago2 protein levels, suggesting TRIM71 can regulate Ago2 turnover. Finally, TRIM71 cooperates with the pluripotency factor Lin-28 in regulating let-7. |
研究领域Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
预测分子量93 kDa
翻译后修饰Autoubiquitinated.
检测图片 (3)
  • ARG54665 anti-TRIM71 antibody ICC/IF image

    Immunofluorescence: human brain cells stained with ARG54665 anti-TRIM71 antibody at 20 μg/ml.

  • ARG54665 anti-TRIM71 antibody IHC image

    Immunohistochemistry: human brain tissue stained with ARG54665 anti-TRIM71 antibody at 5 μg/ml.

  • ARG54665 anti-TRIM71 antibody WB image

    Western blot: human brain tissue lysate stained with ARG54665 anti-TRIM71 antibody at (A) 1 and (B) 2 μg/ml.

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