anti-TRIM71 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes TRIM71
  • 反应物种: Hu, Ms
  • 预测物种: Cow, Rat, Dog
  • 应用: IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: TRIM71
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG65217
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes TRIM71
反应物种Hu, Ms
预测物种Cow, Rat, Dog
应用IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称TRIM71
抗原物种Human
抗原C-RQVKAKSLYLQVE
偶联标记Un-conjugated
別名EC 6.3.2.-; Tripartite motif-containing protein 71; Protein lin-41 homolog; LIN-41; LIN41; E3 ubiquitin-protein ligase TRIM71
应用说明
应用建议
应用推荐稀释比
IHC-P3 - 5 µg/ml
WB0.3 - 1 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 131405 Human TRIM71

GeneID: 636931 Mouse TRIM71

Swiss-port # Q1PSW8 Mouse E3 ubiquitin-protein ligase TRIM71

Swiss-port # Q2Q1W2 Human E3 ubiquitin-protein ligase TRIM71

基因名称TRIM71
全名tripartite motif containing 71, E3 ubiquitin protein ligase
生物功能E3 ubiquitin-protein ligase that cooperates with the microRNAs (miRNAs) machinery and promotes embryonic stem cells proliferation and maintenance. Binds to miRNAs and associates with AGO2, participating in post-transcriptional repression of transcripts such as CDKN1A. Facilitates the G1-S transition to promote rapid embryonic stem cell self-renewal by repressing CDKN1A expression. Required to maintain proliferation and prevent premature differentiation of neural progenitor cells during early neural development: positively regulates FGF signaling by controlling the stability of SHCBP1 (By similarity). [UniProt]
研究领域Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody
预测分子量93 kDa
翻译后修饰Autoubiquitinated.
检测图片 (2)
  • ARG65217 anti-TRIM71 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cerebellum tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65217 anti-TRIM71 antibody at 3.75 µg/ml dilution followed by AP-staining.

  • ARG65217 anti-TRIM71 antibody WB image

    Western blot: 35 µg of HEK293 (A) and NIH/3T3 (B) cell lysates (in RIPA buffer) stained with ARG65217 anti-TRIM71 antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.

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