Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [Clone RM155]; H3K36me3; Histone H3 (trimethyl K36); ChIP Validated; 31-1051-00; 31-1051-00-S
![Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [Clone RM155]; H3K36me3; Histone H3 (trimethyl K36); ChIP Validated; 31-1051-00; 31-1051-00-S](/upload/image/products/Fig-2_RM155.png)
![Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [Clone RM155]; H3K36me3; Histone H3 (trimethyl K36); ChIP Validated; 31-1051-00; 31-1051-00-S](/upload/image/products/Fig-1_RM155.png)
![Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody [Clone RM155]; H3K36me3; Histone H3 (trimethyl K36); ChIP Validated; 31-1051-00; 31-1051-00-S](/upload/image/products/Fig-3_RM155_e.png)
Key features and details
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Product Details
| Product Name | Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM155 |
| Product Description | Rabbit monoclonal to Trimethylated Histone H3 Lysine 36, H3K36me3, Histone H3 (trimethyl K36) |
| Catalog No. | 31-1051-00 |
| Clone Name | RM155 |
| Product Data Sheet | Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody Data Sheet RM155 |
| Specificity | RM155 reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with monomethylated Lysine 36 (K36me1), dimethylated Lysine 36 (K36me2), or other methylations in histone H3. |
| Immunogen | A trimethyl-peptide corresponding to trimethyl-Histone H3 (Lys36). |
| Application | Western Blot (WB) Chromatin IP (ChIP) ELISA Multiplex Immunohistochemistry (IHC) |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL – 2 µg/mL; ChIP: 2 µg/mL - 10 µg/mL; ELISA: 0.2 µg/mL - 1 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL. IHC: 0.1 µg/mL – 0.5 µg/mL. |
| Keywords | |
| References for Trimethyl-Histone H3 (Lys36) antibody [RM155] | Cheung P et al. Single-Cell Chromatin Modification Profiling Reveals Increased Epigenetic Variations with Aging. Cell. (2018) 10.1016/j.cell.2018.03.079. Mass Cytometry; Human.Read more (PubMed: 29706550) |
 | RM155 specifically reacts to Histone H3 trimethylated at Lysine 36 (K36me3). No cross reactivity with non-modified Lysine 36 (K36 Ctrl), monomethylated Lysine 36 (K36me1) or dimethylated Lysine 36 (K36me2), or other methylations in Histone H3. |
 | Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM155 at 2.0 µg/mL, showed a band of histone H3 trimethylated at Lysine 36 (K36me3) in HeLa cells. |
 | ChIP performed on HeLa cells using H3K36me3 antibody (RM155, 5 µg). Real-time PCR was performed using primersspecific to the gene indicated. |
 | Immunocytochemical staining of human normal brain tissue, using Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM155 |
 | Immunocytochemical staining of human normal colon tissue, using Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM155 |
 | Immunocytochemical staining of human breast cancer tissue, using Anti-Trimethyl-Histone H3 (Lys36) Rabbit Monoclonal Antibody, Clone RM155 |
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