Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM137]; H3K4me3; Histone H3 (trimethyl K4); 31-1039-00; 31-1039-00-S
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![Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM137]; H3K4me3; Histone H3 (trimethyl K4); 31-1039-00; 31-1039-00-S](/upload/image/products/RM137.png)
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CAT.NO. : 31-1039-00
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Product Details
Product Details
| Product Name | Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM137 |
| Product Description | Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4) |
| Catalog No. | 31-1039-00 |
| Clone Name | RM137 |
| Product Data Sheet | Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM137 |
| Specificity | RM137 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3. |
| Immunogen | A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4). |
| Application | Western Blot ELISA Multiplex Immunohistochemistry |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.2 µg/mL – 1 µg/mL; ELISA: 0.1 µg/mL – 0.5 µg/mL; Multiplex: 0.1 µg/mL – 0.5 µg/mL; IHC: 0.1 µg/mL – 0.2 µg/mL |
| References for Trimethyl-Histone H3 (Lys4) antibody [RM137] | Shah RN et al Examining the Roles of H3K4 Methylation States with Systematically Characterized Antibodies. Mol Cell. (2018), 10.1016/j.molcel.2018.08.015. ICeChIP. Read more (PubMed: 30244833) Lu P et al. Genome encode analyses reveal the basis of convergent evolution of fleshy fruit ripening. Nat Plants. (2018) 10.1038/s41477-018-0249-z. ChIP; Plant. Read more (PubMed: 30250279) |
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Product Images
 | A Peptide dotblot shows RM137 reacts only to Histone H3 trimethyl-Lysine 4 (K4me3). No cross reactivity with nonmodified Lysine 4 (H3N1-19), monomethylated Lysine 4 (K4me1) or dimethylated Lysine 4 (K4me2). |
 | RM137 specifically reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with other methylated lysines in Histone H3. |
 | Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM137 at 0.5 µg/mL, showed a band of histone H3 trimethylated at Lysine 4 (K4me3) in HeLa cells. |
 | Immunohistochemical staining of formalin fixed and paraffin embedded human Stomach tissue sections, using Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM137. |
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