Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM340]; H3K4me3, Histone H3 (trimethyl K4); ChIP Validated 31-1226-00
![Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM340]; H3K4me3, Histone H3 (trimethyl K4); ChIP Validated 31-1226-00](/upload/image/products/Fig1_H3K4m3ChIP_E.png)
![Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [Clone RM340]; H3K4me3, Histone H3 (trimethyl K4); ChIP Validated 31-1226-00](/upload/image/products/Fig2_H3K4m3ChIP_ELISA_E.png)
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CAT.NO. : 31-1226-00
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Product Details
Product Details
| Product Name | Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM340 |
| Product Description | Rabbit monoclonal to Trimethylated Histone H3 Lysine 4, H3K4me3, Histone H3 (trimethyl K4) |
| Catalog No. | 31-1226-00 |
| Clone Name | RM340 |
| Product Data Sheet | Anti-Trimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM340 |
| Specificity | RM340 reacts to Histone H3 trimethylated at Lysine 4 (K4me3). No cross reactivity with monomethylated Lysine 4 (K4me1), dimethylated Lysine 4 (K4me3), or other methylations in histone H3. |
| Immunogen | A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys4). |
| Application | Chromatin IP (ChIP) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | "ChIP: 1 µg/mL – 5 µg/mL; ELISA: 1 µg/mL – 10 µg/mL; Multiplex: 0.5 µg/mL – 2 µg/mL. |
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Product Images
 | SNAP-ChIP TM / qPCR using anti-H3K4me3 rabbit monoclonal antibody, Clone RM340. Antibody (3 μg at17 μg/mL) was tested in native ChIP with 3 μg HEK- 293 chromatin (~1x106 cells). Specificity (left Y-axis; all bars mean ± SEM from six independent ChIP experiments; note |
 | ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4 Modified), using anti-H3K4me3 rabbit monoclonal antibody, Clone RM340 (10 μg/mL). |
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