Anti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, [Clone RM389], 31-1275-00; H3K9me3, Histone H3 (trimethyl K9)
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CAT.NO. : 31-1275-00
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Product Details
Product Details
| Product Name | Anti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM389 |
| Product Description | Rabbit monoclonal to Trimethylated Histone H3 Lysine 9, H3K9me3, Histone H3 (trimethyl K9) |
| Catalog No. | 31-1275-00 |
| Clone Name | RM389 |
| Product Data Sheet | Anti-Trimethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody Data Sheet RM389 |
| Specificity | RM389 reacts to Histone H3 trimethylated at Lysine 9 (K9me3). No cross reactivity with monomethylated Lysine 9 (K9me1), dimethylated Lysine 9 (K9me2), or other methylations in histone H3. |
| Immunogen | A trimethyl-peptide corresponding to Trimethyl-Histone H3 (Lys9). |
| Application | Western Blot (WB) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt. |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 µg/mL - 2 µg/mL; ELISA: 1 µg/mL - 10 µg/mL; Multiplex: 0.5 µg/mL - 2 µg/mL. |
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Product Images
 | Western Blot of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2), using RM389 at 0.5 µg/mL, showed a band of histone H3 trimethylated at Lysine 9 (K9me3) in HeLa cells. |
 | ELISA of Designer Nucleosomes (dNucs) (Recombinant Human Nucleosome with H3 K4, 9, 27, 36 and H4 K20 Mono-, Di-, and Tri-Methylation), using anti-H3K9me3 rabbit monoclonal antibody, Clone RM389 (0.5 μg/mL). |
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