Anti-Trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody [Clone RM208]; H4K20me3; Histone H4 (Trimethyl Lys20); 31-1096-00; 31-1096-00-S
![Anti-Trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody [Clone RM208]; H4K20me3; Histone H4 (Trimethyl Lys20); 31-1096-00; 31-1096-00-S](/upload/image/products/Fig-2_RM208_H4K20m3_e.png)
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CAT.NO. : 31-1096-00
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Product Details
Product Details
| Product Name | Anti-Trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody, Clone RM208 |
| Product Description | Rabbit monoclonal to Trimethylated Histone H4 Lysine 20, H4K20me3, Histone H4 (Trimethyl K20) |
| Catalog No. | 31-1096-00 |
| Clone Name | RM208 |
| Product Data Sheet | Anti-Trimethyl-Histone H4 (Lys20) Rabbit Monoclonal Antibody Data Sheet RM208 |
| Specificity | RM208 reacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4. |
| Immunogen | A trimethyl-peptide corresponding to trimethyl-Histone H4 (Lys20). |
| Application | Western Blot (WB) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 μg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 1 μg/mL – 2 μg/mL; ELISA: 0.5 μg/mL - 1 μg/mL; Multiplex: 0.1 μg/mL – 0.5 μg/mL. |
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Product Images
 | RM208 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4. |
 | Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM208 at 1 μg/mL, showed a band of Histone H4 trimethylated at Lysine20 in HeLa cells. |
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