anti-Triosephosphate isomerase antibody
| 产品描述 | Goat Polyclonal antibody recognizes Triosephosphate isomerase |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Cow, Rat, Dog |
| 应用 | IHC-P, WB |
| 特异性 | This antibody is expected to recognise both reported isoforms. |
| 宿主 | Goat |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | Triosephosphate isomerase |
| 抗原物种 | Human |
| 抗原 | C-LKPEFVDIINAKQ |
| 偶联标记 | Un-conjugated |
| 別名 | EC 5.3.1.1; HEL-S-49; TPID; TPI; TIM; Triosephosphate isomerase; Triose-phosphate isomerase |
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| 缓冲液 | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 0.5% BSA |
| 浓度 | 0.5 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | |
|---|---|
| 背景介绍 | This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| 研究领域 | Cancer antibody; Metabolism antibody; Signaling Transduction antibody |
| 预测分子量 | 31 kDa |
| 翻译后修饰 | The initiator methionine for isoform 2 is removed. |
ARG63731 anti-Triosephosphate isomerase antibody WB image
Western Blot: human liver lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63731 anti-Triosephosphate isomerase antibody at 0.003 µg/ml dilution.
ARG63731 anti-Triosephosphate isomerase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63731 anti-Triosephosphate isomerase antibody at 5 µg/ml dilution followed by AP-staining.
ARG63731 anti-Triosephosphate isomerase antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Liver (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63731 anti-Triosephosphate isomerase antibody at 2 µg/ml dilution followed by HRP-staining.
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