anti-Triosephosphate isomerase antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes Triosephosphate isomerase
  • 反应物种: Hu, Ms
  • 预测物种: Cow, Rat, Dog
  • 应用: IHC-P, WB
  • 特异性: This antibody is expected to recognise both reported isoforms.
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: Triosephosphate isomerase
  • Brand:
CAT.NO. : ARG63731
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes Triosephosphate isomerase
反应物种Hu, Ms
预测物种Cow, Rat, Dog
应用IHC-P, WB
特异性This antibody is expected to recognise both reported isoforms.
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称Triosephosphate isomerase
抗原物种Human
抗原C-LKPEFVDIINAKQ
偶联标记Un-conjugated
別名EC 5.3.1.1; HEL-S-49; TPID; TPI; TIM; Triosephosphate isomerase; Triose-phosphate isomerase
应用说明
应用建议
应用推荐稀释比
IHC-P2 - 5 µg/ml
WB0.01 - 0.03 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 21991 Mouse TPI1

GeneID: 7167 Human TPI1

Swiss-port # P17751 Mouse Triosephosphate isomerase

Swiss-port # P60174 Human Triosephosphate isomerase

背景介绍This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
研究领域Cancer antibody; Metabolism antibody; Signaling Transduction antibody
预测分子量31 kDa
翻译后修饰The initiator methionine for isoform 2 is removed.
检测图片 (3)
  • ARG63731 anti-Triosephosphate isomerase antibody WB image

    Western Blot: human liver lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63731 anti-Triosephosphate isomerase antibody at 0.003 µg/ml dilution.

  • ARG63731 anti-Triosephosphate isomerase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human pancreas tissue. Antigen Retrieval: Steam tissue section in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG63731 anti-Triosephosphate isomerase antibody at 5 µg/ml dilution followed by AP-staining.

  • ARG63731 anti-Triosephosphate isomerase antibody IHC-P image

    Immunohistochemistry: paraffin embedded Human Liver (Steamed antigen retrieval with Tris/EDTA buffer pH 9) stained with ARG63731 anti-Triosephosphate isomerase antibody at 2 µg/ml dilution followed by HRP-staining.

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