anti-TROP2 antibody [M005]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [M005] recognizes TROP2
  • 反应物种: Hu
  • 应用: ELISA, ICC/IF, IP, WB
  • 特异性: This antibody detects a 50-65 kDa band corresponding to the molecular mass of TROP2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured TROP2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation, as well as for detecting live unfixed cells.
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: M005
  • 同位型: IgG1
  • 靶点名称: TROP2
  • Brand:
CAT.NO. : ARG23866
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Product Details
概述
产品描述Mouse Monoclonal antibody [M005] recognizes TROP2
反应物种Hu
应用ELISA, ICC/IF, IP, WB
特异性This antibody detects a 50-65 kDa band corresponding to the molecular mass of TROP2 on SDS-PAGE immunoblots of native human A431, MDA-MB-231, and MCF-7 cell lysates. The antibody also detects denatured TROP2, but with less affinity. Lower dilutions of the antibody should be used for denatured western blot. Clone M005 can be used for western blot, immunocytochemistry, ELISA, and immunoprecipitation, as well as for detecting live unfixed cells.
宿主Mouse
克隆Monoclonal
克隆号M005
同位型IgG1
靶点名称TROP2
抗原物种Human
抗原Extracellular region of Human TROP2 from the A431 epidermoid carcinoma cell line.
偶联标记Un-conjugated
別名Membrane component chromosome 1 surface marker 1; M1S1; Cell surface glycoprotein Trop-2; TROP2; EGP-1; EGP1; Tumor-associated calcium signal transducer 2; GA733-1; Pancreatic carcinoma marker protein GA733-1; GA7331; GP50
应用说明
应用建议
应用推荐稀释比
ELISA1:2000
ICC/IF1:100
IP1:100
WB1:1000
应用说明WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
实际分子量50 - 65 kDa
属性
形式Liquid
纯化Purification with Protein G.
缓冲液PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
抗菌剂0.05% Sodium azide
稳定剂50% Glycerol and 1 mg/ml BSA
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 4070 Human TACSTD2

Swiss-port # P09758 Human Tumor-associated calcium signal transducer 2

基因名称TACSTD2
全名tumor-associated calcium signal transducer 2
背景介绍This intronless gene encodes a carcinoma-associated antigen. This antigen is a cell surface receptor that transduces calcium signals. Mutations of this gene have been associated with gelatinous drop-like corneal dystrophy.[provided by RefSeq, Dec 2009]
生物功能May function as a growth factor receptor. [UniProt]
预测分子量36 kDa
翻译后修饰The N-terminus is blocked. [UniProt]
检测图片 (2)
  • ARG23866 anti-TROP2 antibody [M005] ICC/IF image

    Immunofluorescence: Paraformaldehyde-fixed A431 cells stained with ARG23866 anti-TROP2 antibody [M005] (red).

  • ARG23866 anti-TROP2 antibody [M005] WB image

    Western blot: A431, MDA-MB-231 and MCF-7 cell lysates under native ("n") and denatured ("d") conditions. The blots were stained with ARG23866 anti-TROP2 antibody [M005] at 1:1000 dilution.

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