anti-U2AF1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes U2AF1
  • 反应物种: Hu, Ms, Rat
  • 应用: IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: U2AF1
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 1-240 of Human U2AF1 (NP_006749.1).
  • Brand:
CAT.NO. : ARG59764
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes U2AF1
反应物种Hu, Ms, Rat
应用IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称U2AF1
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 1-240 of Human U2AF1 (NP_006749.1).
偶联标记Un-conjugated
別名RN; FP793; U2AF35; U2AFBP; RNU2AF1
应用说明
应用建议
应用推荐稀释比
IHC-P1:50 - 1:200
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照Mouse spleen and 22Rv1
实际分子量38 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 108121 Mouse U2AF1

GeneID: 7307 Human U2AF1

Swiss-port # Q01081 Human Splicing factor U2AF 35 kDa subunit

Swiss-port # Q9D883 Mouse Splicing factor U2AF 35 kDa subunit

基因名称U2AF1
全名U2 small nuclear RNA auxiliary factor 1
背景介绍This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
生物功能Plays a critical role in both constitutive and enhancer-dependent splicing by mediating protein-protein interactions and protein-RNA interactions required for accurate 3'-splice site selection. Recruits U2 snRNP to the branch point. Directly mediates interactions between U2AF2 and proteins bound to the enhancers and thus may function as a bridge between U2AF2 and the enhancer complex to recruit it to the adjacent intron. [UniProt]
细胞定位Nucleus. Nucleus speckle. [UniProt]
预测分子量28 kDa
检测图片 (3)
  • ARG59764 anti-U2AF1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat intestine stained with ARG59764 anti-U2AF1 antibody at 1:200 dilution.

  • ARG59764 anti-U2AF1 antibody WB image

    Western blot: 25 µg of Mouse spleen and 22Rv1 cell lysates stained with ARG59764 anti-U2AF1 antibody at 1:1000 dilution.

  • ARG59764 anti-U2AF1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human oophoroma stained with ARG59764 anti-U2AF1 antibody at 1:200 dilution.

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