anti-UBE2F / NCE2 antibody

Key features and details

  • 产品描述: Goat Polyclonal antibody recognizes UBE2F / NCE2
  • 反应物种: Hu, Ms
  • 预测物种: Cow, Rat, Dog
  • 应用: IHC-P, WB
  • 宿主: Goat
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: UBE2F / NCE2
  • 抗原物种: Human
  • Brand:
CAT.NO. : ARG64919
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Product Details
概述
产品描述Goat Polyclonal antibody recognizes UBE2F / NCE2
反应物种Hu, Ms
预测物种Cow, Rat, Dog
应用IHC-P, WB
宿主Goat
克隆Polyclonal
同位型IgG
靶点名称UBE2F / NCE2
抗原物种Human
抗原C-HLRDKEDFRNKVDD
偶联标记Un-conjugated
別名NEDD8-conjugating enzyme 2; EC 6.3.2.-; NEDD8-conjugating enzyme UBE2F; Ubiquitin-conjugating enzyme E2 F; NEDD8 carrier protein UBE2F; NCE2; NEDD8 protein ligase UBE2F
应用说明
应用建议
应用推荐稀释比
IHC-P2 - 4 µg/ml
WB0.05 - 0.2 µg/ml
应用说明WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
缓冲液Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
抗菌剂0.02% Sodium azide
稳定剂0.5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 140739 Human UBE2F

GeneID: 67921 Mouse UBE2F

Swiss-port # Q969M7 Human NEDD8-conjugating enzyme UBE2F

Swiss-port # Q9CY34 Mouse NEDD8-conjugating enzyme UBE2F

基因名称UBE2F
全名ubiquitin-conjugating enzyme E2F (putative)
生物功能Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. [UniProt]
研究领域Cell Biology and Cellular Response antibody; Gene Regulation antibody
预测分子量21 kDa
翻译后修饰The acetylation of Met-1 increases affinity for DCUN1D3 by about 2 orders of magnitude and is crucial for NEDD8 transfer to cullins.
检测图片 (2)
  • ARG64919 anti-UBE2F / NCE2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human placenta tissue stained with ARG64919 anti-UBE2F / NCE2 antibody shows membrane staining in trophoblasts. Recommended concentration: 2.5 - 3.8 µg/ml dilution.

  • ARG64919 anti-UBE2F / NCE2 antibody WB image

    Western blot: 35 µg of Mouse liver lysate (in RIPA buffer) stained with ARG64919 anti-UBE2F / NCE2 antibody at 0.05 µg/ml dilution and incubated at RT for 1 hour.

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