anti-UBE2N / UBC13 antibody
Key features and details
- 产品描述:
- 反应物种:
- 预测物种:
- 应用:
- 宿主:
- 克隆:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
CAT.NO. : ARG43191
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Product Details
概述
| 产品描述 | Rabbit Polyclonal antibody recognizes UBE2N / UBC13 |
|---|---|
| 反应物种 | Hu, Ms |
| 预测物种 | Rat |
| 应用 | ICC/IF, IHC-P, WB |
| 宿主 | Rabbit |
| 克隆 | Polyclonal |
| 同位型 | IgG |
| 靶点名称 | UBE2N / UBC13 |
| 抗原物种 | Human |
| 抗原 | A 15-amino acid peptide within the last 50 amino acids of Human UBE2N / UBC13. |
| 偶联标记 | Un-conjugated |
| 別名 | Ubiquitin-conjugating enzyme E2 N; Ubiquitin-protein ligase N; EC 6.3.2.19; HEL-S-71; Ubc13; Bendless-like ubiquitin-conjugating enzyme; Ubiquitin carrier protein N; UBC13; UbcH13; UBCHBEN; UbcH-ben |
应用说明
| 应用建议 |
| ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| 阳性对照 | Human small intestine cells | ||||||||
| 实际分子量 | ~ 16 kDa |
属性
| 形式 | Liquid |
|---|---|
| 纯化 | Purified by affinity chromatography. |
| 缓冲液 | PBS and 0.02% Sodium azide. |
| 抗菌剂 | 0.02% Sodium azide |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | UBE2N |
| 全名 | ubiquitin-conjugating enzyme E2N |
| 背景介绍 | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq, Jul 2008] |
| 生物功能 | The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIG-I/DDX58 to activate the downstream signaling pathway that leads to interferon beta production (PubMed:28469175, PubMed:31006531). [UniProt] |
| 细胞定位 | Nucleus. Cytoplasm. [UniProt] |
| 预测分子量 | 17 kDa |
| 翻译后修饰 | Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. [UniProt] |
检测图片 (4)
ARG43191 anti-UBE2N / UBC13 antibody ICC/IF image
Immunofluorescence: Mouse thymus cells stained with ARG43191 anti-UBE2N / UBC13 antibody at 10 µg/ml dilution.
ARG43191 anti-UBE2N / UBC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse thymus tissue stained with ARG43191 anti-UBE2N / UBC13 antibody at 2 µg/ml dilution.
ARG43191 anti-UBE2N / UBC13 antibody WB image
Western blot: Human small intestine cell lysate stained with ARG43191 anti-UBE2N / UBC13 antibody at 0.5, 1 and 2 µg/ml dilution.
ARG43191 anti-UBE2N / UBC13 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse thymus tissue stained with ARG43191 anti-UBE2N / UBC13 antibody at 2.5 µg/ml dilution.
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