anti-UCHL1 / PGP9.5 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes UCHL1 / PGP9.5
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, ICC/IF, IHC-P, IP, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: UCHL1 / PGP9.5
  • 抗原物种: Human
  • 抗原: Synthetic peptide derived from Human UCHL1 / PGP9.5.
  • Brand:
CAT.NO. : ARG43278
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes UCHL1 / PGP9.5
反应物种Hu, Ms, Rat
应用FACS, ICC/IF, IHC-P, IP, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称UCHL1 / PGP9.5
抗原物种Human
抗原Synthetic peptide derived from Human UCHL1 / PGP9.5.
偶联标记Un-conjugated
別名PGP95; UCH-L1; PGP9.5; PARK5; Ubiquitin thioesterase L1; HEL-117; Neuron cytoplasmic protein 9.5; Uch-L1; EC 6.-.-.-; PGP 9.5; Ubiquitin carboxyl-terminal hydrolase isozyme L1; NDGOA; EC 3.4.19.12
应用说明
应用建议
应用推荐稀释比
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照293T
实际分子量~ 25 kDa
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 22223 Mouse UCHL1

GeneID: 29545 Rat UCHL1

GeneID: 7345 Human UCHL1

基因名称UCHL1
全名ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
背景介绍The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease. [provided by RefSeq, Sep 2009]
生物功能Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (PubMed:9774100, PubMed:8639624, PubMed:12408865, PubMed:23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed:12408865). [UniProt]
细胞定位Cytoplasm. Endoplasmic reticulum membrane; Lipid-anchor. Note=About 30% of total UCHL1 is associated with membranes in brain. [UniProt]
预测分子量25 kDa
翻译后修饰O-glycosylated. [UniProt]
检测图片 (3)
  • ARG43278 anti-UCHL1 / PGP9.5 antibody ICC/IF image

    Immunofluorescence: U87-MG cells stained with ARG43278 anti-UCHL1 / PGP9.5 antibody (green).

  • ARG43278 anti-UCHL1 / PGP9.5 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human prostate cancer tissue stained with ARG43278 anti-UCHL1 / PGP9.5 antibody.

  • ARG43278 anti-UCHL1 / PGP9.5 antibody WB image

    Western blot: 293T cell lysate stained with ARG43278 anti-UCHL1 / PGP9.5 antibody.

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