anti-USO1 antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes USO1
  • 反应物种: Hu, Rat
  • 应用: ICC/IF, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: USO1
  • 抗原物种: Human
  • 抗原: Recombinant fusion protein corresponding to aa. 663-962 of Human USO1 (NP_003706.2).
  • Brand:
CAT.NO. : ARG40759
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Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes USO1
反应物种Hu, Rat
应用ICC/IF, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称USO1
抗原物种Human
抗原Recombinant fusion protein corresponding to aa. 663-962 of Human USO1 (NP_003706.2).
偶联标记Un-conjugated
別名Protein USO1 homolog; VDP; TAP; Transcytosis-associated protein; Vesicle-docking protein; P115; General vesicular transport factor p115
应用说明
应用建议
应用推荐稀释比
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
应用说明* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
阳性对照SGC7901
属性
形式Liquid
纯化Affinity purified.
缓冲液PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
抗菌剂0.02% Sodium azide
稳定剂50% Glycerol
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 56042 Rat USO1

GeneID: 8615 Human USO1

Swiss-port # O60763 Human General vesicular transport factor p115

Swiss-port # P41542 Rat General vesicular transport factor p115

基因名称USO1
全名USO1 vesicle transport factor
背景介绍The protein encoded by this gene is a peripheral membrane protein which recycles between the cytosol and the Golgi apparatus during interphase. It is regulated by phosphorylation: dephosphorylated protein associates with the Golgi membrane and dissociates from the membrane upon phosphorylation. Ras-associated protein 1 recruits this protein to coat protein complex II (COPII) vesicles during budding from the endoplasmic reticulum, where it interacts with a set of COPII vesicle-associated SNAREs to form a cis-SNARE complex that promotes targeting to the Golgi apparatus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]
生物功能General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity (By similarity). [UniProt]
细胞定位Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note=Recycles between the cytosol and the Golgi apparatus during interphase. During interphase, the phosphorylated form is found exclusively in cytosol; the unphosphorylated form is associated with Golgi apparatus membranes. [UniProt]
预测分子量108 kDa
翻译后修饰Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation. [UniProt]
检测图片 (2)
  • ARG40759 anti-USO1 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG40759 anti-USO1 antibody at 1:200 dilution.

  • ARG40759 anti-USO1 antibody WB image

    Western blot: 25 µg of SGC7901 cell lysate stained with ARG40759 anti-USO1 antibody at 1:1000 dilution.

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