anti-V5 tag antibody [V5.E10]

Key features and details

  • 产品描述: Mouse Monoclonal antibody [V5.E10] recognizes V5 tag
  • 反应物种: Other
  • 应用: Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
  • 特异性: Recognizes V5-tagged fusion proteins
  • 宿主: Mouse
  • 克隆: Monoclonal
  • 克隆号: V5.E10
  • 同位型: IgG1
  • 靶点名称: V5 tag
  • Brand:
CAT.NO. : ARG62340
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Product Details
概述
产品描述Mouse Monoclonal antibody [V5.E10] recognizes V5 tag
反应物种Other
应用Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
特异性Recognizes V5-tagged fusion proteins
宿主Mouse
克隆Monoclonal
克隆号V5.E10
同位型IgG1
靶点名称V5 tag
抗原物种Others
抗原GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH.
偶联标记Un-conjugated
应用说明
应用建议
应用推荐稀释比
DotAssay-dependent
ELISAAssay-dependent
ICC/IF1:500-1:2000
IHC-Fr1:500-1:2000
IHC-P1:500-1:2000
IPAssay-dependent
WB1:1000-1:3000
应用说明The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Protein A Purified
纯化说明Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor.
缓冲液10mM PBS (pH 7.2) and 0.05% Sodium azide
抗菌剂0.05% Sodium azide
浓度1 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
背景介绍The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Both sequence is rarely be found in other proteins and can be used for protein analysis including binding assay, functional analysis and tracing the protein location in cells when the specific antibodies for the protein-of-interest are unavailable.
产品亮点Related products:
V5 tag antibodies; Anti-Mouse IgG secondary antibodies;
研究领域Controls and Markers antibody; Gene Regulation antibody
检测图片 (2)
  • ARG62340 anti-V5 tag antibody [V5.E10] WB image

    Western blot: 10 µg of 293T and 293T + myc-V5-mCherry cell lysates stained with ARG62340 anti-V5 tag antibody [V5.E10] at 1:1000 dilution.

  • ARG62340 anti-V5 tag antibody [V5.E10] IHC image

    Immunohistochemistry: Zebrafish Embryos fixed with 0.4% Triton X-100 containing 4% paraformaldehyde and then stained with ARG62340 anti-V5 tag antibody [V5.E10] at 1:250 dilution.

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