anti-VAMP2 antibody [3E5]
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Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原物种:
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Brand:
Product Details
| 产品描述 | Mouse Monoclonal antibody [3E5] recognizes VAMP2 |
|---|---|
| 反应物种 | Hu, Rat |
| 应用 | ICC/IF, WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 3E5 |
| 同位型 | IgG1, kappa |
| 靶点名称 | VAMP2 |
| 抗原物种 | Human |
| 抗原 | Recombinant fragment around aa. 1-89 of Human VAMP2. |
| 偶联标记 | Un-conjugated |
| 別名 | SYB2; Synaptobrevin-2; Vesicle-associated membrane protein 2; VAMP-2 |
| 应用建议 |
| ||||||
|---|---|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
| 形式 | Liquid |
|---|---|
| 纯化 | Purification with Protein G. |
| 缓冲液 | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| 抗菌剂 | 0.02% Sodium azide |
| 稳定剂 | 10% Glycerol |
| 浓度 | 1 mg/ml |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 数据库连接 | Swiss-port # P63027 Human Vesicle-associated membrane protein 2 Swiss-port # P63045 Rat Vesicle-associated membrane protein 2 |
|---|---|
| 基因名称 | VAMP2 |
| 全名 | vesicle-associated membrane protein 2 (synaptobrevin 2) |
| 背景介绍 | The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008] |
| 生物功能 | Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1. [UniProt] |
| 预测分子量 | 13 kDa |
| 翻译后修饰 | Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2. |
ARG56935 anti-VAMP2 antibody [3E5] ICC/IF image
Immunoflorescense: U87MG cell line stained with ARG56935 anti-VAMP2 antibody [3E5] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
ARG56935 anti-VAMP2 antibody [3E5] WB image
Western blot: 50 ng of 1) Recombinant Human VAMP1, 2) Recombinant Human VAMP2, and 3) Recombinant Human VAMP3 stained with ARG56935 anti-VAMP2 antibody [3E5] at 1:1000.
ARG56935 anti-VAMP2 antibody [3E5] WB image
Western blot: 50 ng of Mouse brain tissue lysate stained with ARG56935 anti-VAMP2 antibody [3E5] at 1:1000.
ARG56935 anti-VAMP2 antibody [3E5] WB image
Western blot: 20 µg of Rat brain tissue lysate stained with ARG56935 anti-VAMP2 antibody [3E5] at 1:2000.
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