anti-VRK1 antibody
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 宿主:
- 克隆:
- 克隆号:
- 同位型:
- 靶点名称:
- 抗原:
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Brand:
CAT.NO. : ARG55010
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Product Details
概述
| 产品描述 | Mouse Monoclonal antibody recognizes VRK1 |
|---|---|
| 反应物种 | Hu |
| 应用 | WB |
| 宿主 | Mouse |
| 克隆 | Monoclonal |
| 克隆号 | 1015CT2.1.1 |
| 同位型 | IgG1 |
| 靶点名称 | VRK1 |
| 抗原 | Purified His-tagged VRK1 protein. |
| 偶联标记 | Un-conjugated |
| 別名 | Serine/threonine-protein kinase VRK1; EC 2.7.11.1; PCH1A; Vaccinia-related kinase 1; PCH1 |
应用说明
| 应用建议 |
| ||||
|---|---|---|---|---|---|
| 应用说明 | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| 阳性对照 | HeLa |
属性
| 形式 | Liquid |
|---|---|
| 缓冲液 | Crude Ascites and 0.09% (W/V) Sodium azide |
| 抗菌剂 | 0.09% (W/V) Sodium azide |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q99986 Human Serine/threonine-protein kinase VRK1 |
|---|---|
| 基因名称 | VRK1 |
| 全名 | vaccinia related kinase 1 |
| 背景介绍 | This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008] |
| 生物功能 | Serine/threonine kinase involved in Golgi disassembly during the cell cycle: following phosphorylation by PLK3 during mitosis, required to induce Golgi fragmentation. Acts by mediating phosphorylation of downstream target protein. Phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Phosphorylates casein and histone H3. Phosphorylates BANF1: disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. Phosphorylates ATF2 which activates its transcriptional activity. [UniProt] |
| 细胞定位 | Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Dispersed throughout the cell but not located on mitotic spindle or chromatids during mitosis |
| 研究领域 | Cell Biology and Cellular Response antibody; Gene Regulation antibody; Signaling Transduction antibody |
| 预测分子量 | 45 kDa |
| 翻译后修饰 | Autophosphorylated at various serine and threonine residues. Autophosphorylation does not impair its ability to phosphorylate p53/TP53. Phosphorylation by PLK3 leads to induction of Golgi fragmentation during mitosis. |
检测图片 (1)
ARG55010 anti-VRK1 antibody WB image
Western blot: 35 µg of HeLa cell lysate stained with ARG55010 anti-VRK1 antibody.
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