anti-XDH / Xanthine Oxidase antibody

Key features and details

  • 产品描述: Rabbit Polyclonal antibody recognizes XDH / Xanthine Oxidase
  • 反应物种: Hu, Ms, Rat
  • 应用: FACS, IHC-P, WB
  • 宿主: Rabbit
  • 克隆: Polyclonal
  • 同位型: IgG
  • 靶点名称: XDH / Xanthine Oxidase
  • 抗原物种: Human
  • 抗原: Recombinant protein corresponding to T2-K343 of Human XDH / Xanthine Oxidase.
  • Brand:
CAT.NO. : ARG59194
US$ Please choose
US$ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
*产品价格可能会有所调整,请以品牌方官网实时更新的价格为准,以确保准确性。
Product Details
概述
产品描述Rabbit Polyclonal antibody recognizes XDH / Xanthine Oxidase
反应物种Hu, Ms, Rat
应用FACS, IHC-P, WB
宿主Rabbit
克隆Polyclonal
同位型IgG
靶点名称XDH / Xanthine Oxidase
抗原物种Human
抗原Recombinant protein corresponding to T2-K343 of Human XDH / Xanthine Oxidase.
偶联标记Un-conjugated
別名EC 1.17.1.4; XOR; XO; Xanthine dehydrogenase/oxidase [Includes: Xanthine dehydrogenase; XD; EC 1.17.3.2; Xanthine oxidoreductase
应用说明
应用建议
应用推荐稀释比
FACS1:150 - 1:500
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
应用说明IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
属性
形式Liquid
纯化Affinity purification with immunogen.
缓冲液0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
抗菌剂0.05% Sodium azide
稳定剂5% BSA
浓度0.5 mg/ml
存放说明For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 22436 Mouse XDH

GeneID: 497811 Rat XDH

GeneID: 7498 Human XDH

基因名称XDH
全名xanthine dehydrogenase
背景介绍Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism. [provided by RefSeq, Jan 2014]
生物功能Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro). [UniProt]
细胞定位Cytoplasm. Peroxisome. Secreted. [UniProt]
预测分子量146 kDa
翻译后修饰Subject to partial proteolysis; this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O).

Contains sulfhydryl groups that are easily oxidized (in vitro); this alters the enzyme from the dehydrogenase form (D) to the oxidase form (O). [UniProt]
检测图片 (4)
  • ARG59194 anti-XDH / Xanthine Oxidase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59194 anti-XDH / Xanthine Oxidase antibody at 1 µg/ml dilution, overnight at 4°C.

  • ARG59194 anti-XDH / Xanthine Oxidase antibody WB image

    Western blot: 50 µg of samples under reducing conditions. Rat liver and Mouse liver tissue lysates stained with ARG59194 anti-XDH / Xanthine Oxidase antibody at 0.5 µg/ml, overnight at 4°C.

  • ARG59194 anti-XDH / Xanthine Oxidase antibody FACS image

    Flow Cytometry: A431 cells were blocked with 10% normal goat serum and then stained with ARG59194 anti-XDH / Xanthine Oxidase antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.

  • ARG59194 anti-XDH / Xanthine Oxidase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat intestine tissue. Antigen Retrieval: Heat mediated was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59194 anti-XDH / Xanthine Oxidase antibody at 1 µg/ml dilution, overnight at 4°C.

New Products
Get in touch with AREX
Name:*
Tel/Phone:*
Company:*
Email:*
Inquiry:
Captcha*
Submitting your email information means that you are willing to receive email information from AREX regarding technology, applications, products, and events. By clicking on the 'unsubscribe' button in the email or by contacting info@arexbio.com You can unsubscribe at any time by sending an email. Regarding your data usage information, please refer to our privacy policy.
© AREX 2024. All Rights Reserved. | 浙ICP备2024113709号-3 Sitemap