APC Mouse Monoclonal Antibody(ARA727)
Key features and details
- Reactivity:Human, Rat, Mouse
- Application:WB, IF/ICC, IP
- Host:Mouse
- Clonality:Monoclonal
- Target:APC
-
Brand:
CAT.NO. : ARA6514
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Product Details
Background
This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.
Application
|
Application |
Dilution Ratio |
|
WB |
1:500 - 1:1000 |
|
IF/ICC |
1:50 - 1:100 |
|
IP |
1:10 - 1:50 |
Overview
|
Product Description |
Rabbit monoclonal antibody to APC |
|
Immunogen |
A synthetic peptide of human APC |
|
Purification Method |
The antibody was purified by immunogen affinity chromatography. |
|
Clonality |
Monoclonal |
|
Product Form |
Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% BSA. |
|
Gene Name |
APC |
|
Alternative Names |
DP2.5; Adenomatous polyposis coli protein; Protein APC; Deleted in polyposis 2.5 |
|
Human Gene ID |
324 |
|
Rat Gene ID |
24205 |
|
Human Protein ID |
P25054 |
|
Mouse Protein ID |
Q61315 |
|
Rat Protein ID |
P70478 |
Data
Western blot analysis of APC expression in Hela (A), A549 (B), HL60 (C), U2OS (D) whole cell lysates. (Predicted band size: 312 kD; Observed band size: 160 kD)
Immunofluorescent analysis of APC staining in Lncap cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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