Apoptosis Marker Antibody Duo (Caspase3, PARP)
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Product Details
| 货号 | 内含物名称 | 宿主克隆性 | 反应 | 应用 | 包装 |
|---|---|---|---|---|---|
| ARG20001 | anti-Caspase 3 antibody [C33] | Mouse mAb | Hu | WB | 25 μg |
| ARG20041 | anti-PARP (cleaved) antibody | Rabbit pAb | Hu | IHC, WB | 25 μg |
| 产品描述 | The TUNEL assay has been commonly used as a detection method for identifying apoptotic cells. While useful, it can also yield highly variable results as some non-apoptotic nuclei showing high levels of RNA synthesis/splicing can also be labeled by TUNEL technique. Cleavage of Caspase 3 and PARP (Poly (ADP-ribose) Polymerase is a pre-requisite for cells who undergo final stage of cell death. The use of anti-cleaved caspase3 (ARG20003) and anti-cleaved PARP (ARG20041) antibodies adds a more specific weapon to the arsenal of techniques for confirming apoptosis in tissue specimens. |
|---|---|
| 靶点名称 | Apoptosis Marker |
| 別名 | Apoptosis Marker antibody; Caspase 3 antibody; PARP (cleaved) antibody |
| 存放说明 | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
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| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
| 全名 | Antibody Duo for Apoptosis Marker (Caspase3, PARP) |
|---|---|
| 研究领域 | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Neuroscience antibody |
ARG20001 anti-Caspase 3 antibody [C33] WB image
Western blot: 30 µg of HeLa cells untreated or treated with Staurosporine (1μM, overnight). The blots were stained with ARG20001 anti-Caspase 3 antibody [C33] at 1:500 dilution.
ARG20041 anti-PARP (cleaved) antibody WB image
Western blot: 30 µg of HeLa untreated or treated with Staurosporine and stained with ARG20041 anti-PARP (cleaved) antibody at 1:500 dilution.
ARG20001 anti-Caspase-3 antibody [C33] WB image
Western blot: HeLa cells treated with camptothecin (2 µM) stained with ARG20001 anti-Caspase-3 antibody [C33].
The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. However, the optimal conditions should be determined individually.ARG20001 anti-Caspase 3 antibody [C33] WB image
Western blot: 20 µg of HeLa cell lysates stained with ARG20001 anti-Caspase 3 antibody [C33] at 2 µg/ml dilution.
Moonlighting glyceraldehyde-3-phosphate dehydrogenase of Lactobacillus gasseri inhibits keratinocyte apoptosis and skin inflammation in experimental atopic dermatitis
ARG20001: WB / Mouse
Inhibition of histone acetylation by curcumin reduces alcohol-induced fetal cardiac apoptosis.
ARG20001: WB / Mouse
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