Beta Actin Rabbit Monoclonal antibody(ARA710)
Key features and details
- Reactivity:Human, Mouse, Rat, Pig
- Application:WB, IHC, IF/ICC, IP
- Host:Rabbit
- Clonality:Monoclonal
- lsotype: IgG
- Target:Beta Actin
-
Brand:
CAT.NO. : ARA6450
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
Background
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Application
|
Application |
Dilution Ratio |
|
WB |
1:5000 - 1:40000 |
|
IHC |
1:1000 - 1:5000 |
|
IF/ICC |
1:500 - 1:4000 |
|
IP |
0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Overview
|
Reactivity |
Human, Mouse, Rat, Pig |
|
Host / Isotype |
Rabbit / IgG |
|
Class |
Recombinant |
|
Type |
Antibody |
|
Immunogen |
Recombinant human beta actin protein |
|
Full Name |
actin, beta |
|
Calculated Molecular Weight |
375 aa, 42 kDa |
|
Observed Molecular Weight |
42 kDa |
|
GenBank Accession Number |
BC002409 |
|
Gene Symbol |
Beta Actin |
|
Gene ID (NCBI) |
60 |
|
RRID |
AB_2923704 |
|
Conjugate |
Unconjugated |
|
Form |
Liquid |
|
Purification Method |
Protein A purification |
|
UNIPROT ID |
P60709 |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Data
Various lysates were subjected to SDS PAGE followed by western blot with Beta Actin Rabbit Monoclonal antibody(ARA710) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using Beta Actin Rabbit Monoclonal antibody(ARA710) at dilution of 1:2000 . Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using Beta Actin Rabbit Monoclonal antibody(ARA710) at dilution of 1:1900 and Fluor 488-Conjugated AffiniPure Rabbit IgG(H+L) Goat Antibody.
IP result of Beta Actin Rabbit Monoclonal antibody(ARA710) (IP:4ug; Detection:1:5000) with HeLa cells lysate 1400 ug.
Storage
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
New Products
