Beta III Tubulin Mouse Monoclonal Antibody(ARA835)
Key features and details
- Reactivity:Human, Mouse, Rat
- Application:WB, IF, IHC-P, FC
- Host: Mouse
- Clonality:Monoclonal
- Target:Beta III Tubulin
-
Brand:
CAT.NO. : ARA6649
US＄ Please choose
US＄ Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
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Product Details
Background
Tubulin is a compound of subunits of A tubulin and B tubulin. Class III beta tubulin (beta III-tubulin) is a vertebrate tubulin isotype specific to the neurons and mammalian testis cells, making it an ideal neuronal marker. Overexpression of class III beta tubulin is associated with the resistances of microtubule-targeted cancer drugs in lung cancer cell lines, breast cancer cell lines, and ovarian tumors.
Application
|
Application |
Dilution Ratio |
|
WB |
1:2,000-1:5,000 |
|
IF-Cell |
1:500-1:1,000 |
|
IHC-P |
1:1,500-1:2,000 |
|
FC |
1:800-1:1,000 |
Overview
|
Product Name |
Beta III Tubulin Mouse Monoclonal Antibody (ARA835) |
|
Antibody Type |
Mouse Monoclonal Antibody |
|
Immunogen |
Synthetic peptide (KLH-coupled) within human Tubulin beta-3 chain aa 401-450. |
|
Species Reactivity |
Human, Mouse, Rat |
|
Validated Applications |
WB, IF-Cell, IHC-P, FC, IF-Tissue |
|
Molecular Weight |
Predicted band size: 50 kDa |
|
Positive Control |
SH-SY5Y cell lysate, U-87 MG cell lysate, A-172 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, HEK-293, SH-SY5Y, PC-12, Neuro-2a, human brain tissue, mouse brain tissue, rat brain tissue, mouse hippocampus tissue, MCF7. |
|
Conjugation |
unconjugated |
Data
Western blot analysis of Beta III Tubulin on different lysates with Mouse anti-Beta III Tubulin antibody (ARA835) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Beta III Tubulin
antibody (ARA835) at 1/2,000 dilution.
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Beta III Tubulin antibody (ARA835) at 1/2,000 dilution.
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Beta III Tubulin antibody (ARA835) at 1/2,000 dilution.
Storage
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
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