Beta Tubulin Mouse Monoclonal antibody(ARA709)
Key features and details
- Reactivity:Human, Mouse, Rat, Pig, Zebrafish, Nematode
- Application:WB, IHC, IF/ICC, IP, ELISA
- Host:Mouse
- Clonality:Monoclonal
- lsotype: IgG
- Target:Beta Tubulin
-
Brand:
Product Details
|
Application |
Dilution Ratio |
|
WB |
1:10000 - 1:120000 |
|
IP |
0.4-4.0 µg for 1.0-3.0 mg of total protein lysate |
|
IHC |
1:50 - 1:1000 |
|
IF/ICC |
1:200 - 1:1000 |
|
Applications |
WB, IHC, IF/ICC, IP, ELISA |
|
Tested Reactivity |
Human, Mouse, Rat, Pig, Zebrafish, Nematode |
|
Immunogen |
Recombinant human Tubulin-beta protein |
|
Host / Isotype |
Mouse / IgG2a |
|
Antibody Class |
Monoclonal |
|
Product Type |
Antibody |
|
Full Name |
tubulin, beta 3 |
|
Alternative Names |
Tubulin beta 3 chain, Tubulin beta III, 1D4A4, TUBB4, Tubulin beta-3 chain |
|
Calculated Molecular Weight |
450 aa, 50 kDa |
|
Observed Molecular Weight |
50-55 kDa |
|
GenBank Protein Accession |
BC000748 |
|
Gene Symbol |
TUBB3 |
|
Gene ID (NCBI) |
10381 |
|
RRID |
AB_2881629 |
|
Conjugate Type |
Unconjugated |
|
Form |
Liquid |
|
Purification Method |
Protein A purification |
|
UNIPROT ID |
Q13509 |
|
Storage Buffer |
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Various lysates were subjected to SDS PAGE followed by western blot with Beta Tubulin Mouse Monoclonal antibody(ARA709) at dilution of 1:200000 incubated at room temperature for 1.5 hours.
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using Beta Tubulin Mouse Monoclonal antibody(ARA709).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Beta Tubulin Mouse Monoclonal antibody(ARA709) at dilution of 1:400 and Fluor 488-Conjugated AffiniPure Mouse IgG(H+L) Goat antibody, Fluour 594-Phalloidin (red).
IP result of Beta Tubulin Mouse Monoclonal antibody(ARA709) (IP:5ug; Detection:1:1000) with HeLa cells lysate 880ug.
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