Biotin Anti-His-Tag Rabbit Monoclonal Antibody [Clone RM146]; 6x His-Tag or 10x His-Tag; 31-1048-02
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![Biotin Anti-His-Tag Rabbit Monoclonal Antibody [Clone RM146]; 6x His-Tag or 10x His-Tag; 31-1048-02](/upload/image/products/Fig1_ELISA_Biotin-RbtXHisTag_1.png)
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CAT.NO. : 31-1048-02
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Product Details
Product Details
| Product Name | Biotin Anti-His-Tag (6xHis-Tag or 10xHis-Tag) Rabbit Monoclonal Antibody, Clone RM146 |
| Product Description | Biotinylated Rabbit monoclonal to His-Tag. (6xHis-Tag or 10xHis-Tag) at either the N-Terminus (amino) or C-Terminus (carboxy) |
| Catalog No. | 31-1048-02 |
| Clone Name | RM146 |
| Product Data Sheet | Biotin Anti-His Tag Rabbit Monoclonal Antibody Data Sheet RM146 |
| Specificity | RM146 reacts to recombinant proteins containing the 6xHis-Tag or 10xHis-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells. |
| Immunogen | Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus. |
| Application | Western Blot ELISA Immunoprecipitation Immunocytochemistry Flow Cytometry Immunohistochemistry |
| Species Reactivity | All |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.1 µg/mL – 0.5 µg/mL; Immunocytochemistry 0.5 µg/mL – 2 µg/mL; Flow Cytometry: 0.5 µg/mL – 2 µg/mL; Immunohistochemistry; 0.1 µg/mL - 1 µg/mL; ELISA: 0.01 µg/mL-0.5 µg/mL. |
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Product Images
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 | Western blot of 293T cells transfected (+) or untrans-fected (-) with a DNA construct encoding His-Tag Histone H3 (G34W) protein, using Biotin Anti-His-Tag Rabbit Monoclonal Antibody, Clone RM146, at 0.2 µg/mL, followed by a HRP conjugated Streptavidin. |
 | Immunohistochemistry staining of naïve HepG2 cells (Negative control) using anti-His-Tag antibody, Clone RM146. |
 | Immunohistochemistry staining of 293T cells expressing His-Tag nuclear protein X, using anti-His-Tag antibody, Clone RM146. |
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