Biotin Anti-Human IgA1 Rabbit Monoclonal Antibody [Clone RM124], 31-1026-02

Key features and details

  • Uniprot: P01876
  • Gene ID: 3493
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Concentration: 1 mg/mL
  • Brand:
CAT.NO. : 31-1026-02
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Product Details
Product Details
Product NameBiotin Anti-Human IgA1 Rabbit Monoclonal Antibody, RM124
Product DescriptionBiotinylated Rabbit monoclonal to Human IgA1
Catalog No.31-1026-02
Clone NameRM124
Product Data SheetBiotin Anti-Human IgA1 Rabbit Monoclonal Antibody Data Sheet RM124
SpecificityRM124 reacts to human IgA1, and very slightly cross reacts with IgA2. No cross reactivity with human IgG, IgM, IgD, or IgE.
ImmunogenHuman IgA
ApplicationELISA
Immunocytochemistry (ICC)
Immunohistochemistry (IHC)

Species ReactivityHuman
ConjugateBiotin
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Storage Buffer50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size50 µg
Concentration1.0 mg/mL
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
UsageELISA: 50 ng/well – 200 ng/well (for Capture);
0.05 µg/mL – 0.2 µg/mL (for Detection);
Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL;
Immunohistochemistry (IHC): 0.1 µg/mL - 1 µg/mL.
Keywords
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Product Images
Immunohistochemistry

Immunohistochemistry of Human Tonsil Tissue using Anti-Human IgA1 antibody, Clone RM124.

Sandwich

Sandwich ELISA using RM124 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.


ELISA

ELISA of human immunoglobulins shows RM124 reacts only to Human IgA. Very slightly cross reacts with IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

A

A titer ELISA using RM124. The plate was coated with different amounts of human IgA1.A serial dilution of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

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