Biotin Anti-Human IgD Rabbit Monoclonal Antibody [Clone RM123], 31-1025-02
![Biotin Anti-Human IgD Rabbit Monoclonal Antibody [Clone RM123], 31-1025-02](/upload/image/products/Fi1igdg.png)
![Biotin Anti-Human IgD Rabbit Monoclonal Antibody [Clone RM123], 31-1025-02](/upload/image/products/Fig3igd.png)
![Biotin Anti-Human IgD Rabbit Monoclonal Antibody [Clone RM123], 31-1025-02](/upload/image/products/Fig2igd.png)
Key features and details
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Product Details
| Product Name | Biotin Anti-Human IgD Rabbit Monoclonal Antibody, Clone RM123 |
| Product Description | Biotinylated Rabbit monoclonal to Human IgD |
| Catalog No. | 31-1025-02 |
| Clone Name | RM123 |
| Product Data Sheet | Biotin Anti-Human IgD Rabbit Monoclonal Antibody Data Sheet RM123 |
| Specificity | RM123 reacts to human IgD. No cross reactivity with human IgG, IgM, IgE, or IgA. |
| Immunogen | Human IgD |
| Application | ELISA |
| Species Reactivity | Human |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 25 ng/well – 200 ng/well (for Capture); 0.01 µg/mL – 0.1 µg/mL (for Detection); |
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 | Sandwich ELISA using RM123 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin. |
 | ELISA of human immunoglobulins shows RM123 reacts to IgD from human plasma and IgDκ from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA using RM123. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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