Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody [Clone RM117], 31-1019-02
![Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody [Clone RM117], 31-1019-02](/upload/image/products/RM117-Fig3_E.png)
![Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody [Clone RM117], 31-1019-02](/upload/image/products/RM117-Fig2_E.png)
![Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody [Clone RM117], 31-1019-02](/upload/image/products/RM117-Fig1b_E.png)
![Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody [Clone RM117], 31-1019-02](/upload/image/products/RM117-Fig1a_E.png)
Key features and details
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Product Details
| Product Name | Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody, Clone RM117 |
| Product Description | Biotinylated Rabbit monoclonal to Human IgG1 |
| Catalog No. | 31-1019-02 |
| Clone Name | RM117 |
| Product Data Sheet | Biotin Anti-Human IgG1 Rabbit Monoclonal Antibody Data Sheet RM117 |
| Specificity | RM117 reacts to the heavy chain of human IgG1. No cross reactivity with human IgG2, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Peptide corresponding to the hinge region of Human IgG1 |
| Application | Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA |
| Species Reactivity | Human |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection); Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL; Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL; Flow Cytometry (FC): 2 µg/mL - 8 µg/mL. |
| Keywords | |
| . | |
 | Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. |
 | Sandwich ELISA using RM117 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. |
 | ELISA of human immunoglobulins shows RM117 only reacted to human IgG1. No cross reactivity with Human IgG2, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA using RM117. The plate was coated with different amounts of human IgG1.A serial dilution of RM117 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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