Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody [Clone RM118], 31-1020-02
![Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody [Clone RM118], 31-1020-02](/upload/image/products/Fig-3_RM118_e.png)
![Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody [Clone RM118], 31-1020-02](/upload/image/products/Fig-2_RM118_e.png)
![Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody [Clone RM118], 31-1020-02](/upload/image/products/Fig-1b_RM118_e.png)
![Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody [Clone RM118], 31-1020-02](/upload/image/products/Fig-1a_RM118_e.png)
Key features and details
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Brand:
Product Details
| Product Name | Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody, Clone RM118 |
| Product Description | Biotinylated Rabbit monoclonal to Human IgG2 |
| Catalog No. | 31-1020-02 |
| Clone Name | RM118 |
| Product Data Sheet | Biotin Anti-Human IgG2 Rabbit Monoclonal Antibody Data Sheet RM118 |
| Specificity | Rm118 reacts to the heavy chain of human IgG2. No cross reactivity with human IgG1, IgG3, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Human IgG2 |
| Application | ELISA |
| Species Reactivity | Human |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection); |
| Keywords | |
| . | |
 | Sandwich ELISA using RM118 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. |
 | Sandwich ELISA using RM118 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. |
 | ELISA of human immunoglobulins shows RM118 only reacted to human IgG2. No cross reactivity with Human IgG1, IgG3, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA using RM118. The plate was coated with different amounts of human IgG2.A serial dilution of RM118 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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