Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody [Clone RM119], 31-1021-02
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![Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody [Clone RM119], 31-1021-02](/upload/image/products/RM119_image041.png)
![Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody [Clone RM119], 31-1021-02](/upload/image/products/RM119_image021.png)
Key features and details
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Product Details
| Product Name | Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody, Clone RM119 |
| Product Description | Biotinylated Rabbit monoclonal to Human IgG3 |
| Catalog No. | 31-1021-02 |
| Clone Name | RM119 |
| Product Data Sheet | Biotin Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119 |
| Specificity | RM119 reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Human IgG3 |
| Application | ELISA Immunocytochemistry (ICC) Immunohistochemistry (IHC) |
| Species Reactivity | Human |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection); Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL; Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL. |
| Keywords | |
| . | |
 | Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG3 antibody, Clone RM119. |
 | Sandwich ELISA using RM119 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin. |
 | Sandwich ELISA using RM119 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an HRP conjugated streptavidin. |
 | ELISA of human immunoglobulins shows RM119 reacts only to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins.200 ng/mL, 50 ng/mL, or 10 ng/mL of RM119 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA using RM119. The plate was coated with different amounts of human IgG3.A serial dilution of RM124 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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