Biotin Anti-Human IgG4 Fc Rabbit Monoclonal Antibody [Clone RM217], 31-1100-02
![Biotin Anti-Human IgG4 Fc Rabbit Monoclonal Antibody [Clone RM217], 31-1100-02](/upload/image/products/Fig1-HuIgG4Fc_E.png)
![Biotin Anti-Human IgG4 Fc Rabbit Monoclonal Antibody [Clone RM217], 31-1100-02](/upload/image/products/Fig3-HuIgG4Fc_e.png)
![Biotin Anti-Human IgG4 Fc Rabbit Monoclonal Antibody [Clone RM217], 31-1100-02](/upload/image/products/Fig2-HuIgG4Fc_e.png)
Key features and details
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CAT.NO. : 31-1100-02
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Product Details
Product Details
| Product Name | Biotin Anti-Human IgG4 Fc Rabbit Monoclonal Antibody, Clone RM217 |
| Product Description | Biotinylated Rabbit monoclonal to Human IgG4 Fc |
| Catalog No. | 31-1100-02 |
| Clone Name | RM217 |
| Product Data Sheet | Biotin Anti-Human IgG4 Fc Rabbit Monoclonal Antibody Data Sheet RM217 |
| Specificity | RM217 reacts to the Fc region of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Peptide corresponding to the Fc region of Human IgG4 |
| Application | ELISA |
| Species Reactivity | Human |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
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Properties
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection). |
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Product Images
 | RM217 specifically reacts to human IgG4. No cross reactivity with human IgG1, IgG2, IgG3. |
 | Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. |
 | Sandwich ELISA using RM217 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. |
 | A titer ELISA using RM217. The plate was coated with different amounts of human IgG4.A serial dilution of RM217 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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