Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [Clone RM120], 31-1022-02
![Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [Clone RM120], 31-1022-02](/upload/image/products/RM120_Fig4_E.jpg)
![Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [Clone RM120], 31-1022-02](/upload/image/products/RM120-Fig3.png)
![Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [Clone RM120], 31-1022-02](/upload/image/products/RM120-Fig2_E.png)
![Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [Clone RM120], 31-1022-02](/upload/image/products/RM120-Fig1b_E.png)
![Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody [Clone RM120], 31-1022-02](/upload/image/products/RM120-Fig1a_E.png)
Key features and details
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Product Details
| Product Name | Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody, Clone RM120 |
| Product Description | Biotinylated Rabbit monoclonal to Human IgG4 |
| Catalog No. | 31-1022-02 |
| Clone Name | RM120 |
| Product Data Sheet | Biotin Anti-Human IgG4 Rabbit Monoclonal Antibody Data Sheet RM120 |
| Specificity | RM120 reacts to the heavy chain of human IgG4. No cross reactivity with human IgG1, IgG2, IgG3, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Peptide corresponding to the hinge region of Human IgG4 |
| Application | ELISA Western Blot Immunohistochemistry (IHC) Immunocytochemistry (ICC) Flow Cytometry (FC) |
| Species Reactivity | Human |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection); Western Blot (WB): 0.5 µg/mL – 2 µg/mL; Immunocytochemistry (ICC): 1 µg/mL - 10 µg/mL; Immunohistochemistry (IHC): 1 µg/mL - 10 µg/mL; Flow Cytometry (FC): 1 µg/mL - 4 µg/mL. |
| Keywords | |
| . | |
 | Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. |
 | Sandwich ELISA using RM120 as the capture antibody, and Biotinylated anti-human light chains (κ+ λ)antibody RM129 as the detection antibody, followed by an AP conjugated streptavidin. |
 | Western Blot of human, mouse, rat, and goat IgG shows RM120 reacts to human IgG4, in both whole molecule (~150kDa, non-reduced) and heavy chain (~50kDa, reduced) forms. No cross reactivity with other isotypes of human IgG, or mouse, rat, or goat IgG. |
 | A titer ELISA using RM120. The plate was coated with different amounts of human IgG4. A serial dilution of RM120 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | Immunohistochemistry of Human Lymphoid Tissue using Anti-Human IgG4 antibody, Clone RM120. |
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