Biotin Anti-Human IgM Rabbit Monoclonal Antibody [Clone RM121], 31-1023-02
![Biotin Anti-Human IgM Rabbit Monoclonal Antibody [Clone RM121], 31-1023-02](/upload/image/products/RM121_image031.png)
![Biotin Anti-Human IgM Rabbit Monoclonal Antibody [Clone RM121], 31-1023-02](/upload/image/products/RM121_image021.png)
![Biotin Anti-Human IgM Rabbit Monoclonal Antibody [Clone RM121], 31-1023-02](/upload/image/products/RM121_image011.png)
![Biotin Anti-Human IgM Rabbit Monoclonal Antibody [Clone RM121], 31-1023-02](/upload/image/products/RM121_image041.png)
Key features and details
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Product Details
| Product Name | Biotin Anti-Human IgM Rabbit Monoclonal Antibody, Clone RM121 |
| Product Description | Biotinylated Rabbit monoclonal to Human IgM |
| Catalog No. | 31-1023-02 |
| Clone Name | RM121 |
| Product Data Sheet | Biotin Anti-Human IgM Rabbit Monoclonal Antibody Data Sheet RM121 |
| Specificity | RM121 reacts to the heavy chain of human IgM. No cross reactivity with human IgG, IgA, IgD, or IgE. |
| Immunogen | Human IgM |
| Application | ELISA Immunocytochemistry (ICC) Immunohistochemistry (IHC) |
| Species Reactivity | Human |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50 ng/well – 200 ng/well (for Capture); 0.05 µg/mL – 0.2 µg/mL (for Detection); Immunocytochemistry (ICC): 0.5 µg/mL - 2 µg/mL; Immunohistochemistry (IHC): 0.5 µg/mL - 2 µg/mL. |
| Keywords | |
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 | Sandwich ELISA using RM121 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin. |
 | Sandwich ELISA using RM121 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin. |
 | ELISA of human immunoglobulins shows RM121 reacts only to human IgM. No cross reactivity with Human IgG, IgE, IgD, or IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA using RM121. The plate was coated with different amounts of human IgM.A serial dilution of RM121 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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