Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody [Clone RM104], 31-1001-02
![Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody [Clone RM104], 31-1001-02](/upload/image/products/RM104_WB-e1625768142821.png)
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![Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody [Clone RM104], 31-1001-02](/upload/image/products/Anri-mouse-IgG-3.jpg)
Key features and details
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Product Details
| Product Name | Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody, Clone RM104 |
| Product Description | Biotinylated Rabbit monoclonal to Mouse IgG |
| Catalog No. | 31-1001-02 |
| Clone Name | RM104 |
| Product Data Sheet | Biotin Anti-Mouse IgG Rabbit Monoclonal Antibody Data Sheet RM104 |
| Specificity | RM104 reacts to the Fc region of all mouse IgG subclasses. No cross reactivity with mouse IgM, IgA, IgE, human IgG, and rat IgG. It may cross react to goat IgG. The Fc region of RM104 has been engineered to eliminate Fc receptor binding. |
| Immunogen | Mouse IgG |
| Application | ELISA Western Blot (WB) (nonreduced) |
| Species Reactivity | Mouse |
| Conjugate | Biotin |
| Intended Use | Research Use Only |
| . | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 50 µg |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 0.005 µg/mL - 0.2 µg/mL; Western Blot (WB): 0.1 µg/mL - 0.5 µg/mL. |
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 | Western blot of nonreduced (-) and reduced (+) mouse immunoglobulins (20 ng/lane), using 0.2 µg/mL of RevMAb Clone RM104. This antibody reacts to nonreduced mouse IgG1, IgG2a, IgG2b, and IgG3. It showed no cross reactivity with IgM, IgA, or IgE. |
 | ELISA of mouse immunoglobulins shows RM104 reacts to the Fc region of mouse IgG1, IgG2a, IgG2b, and IgG3; no cross reactivity with IgM, IgA, IgE, human or rat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
 | A titer ELISA of mouse IgG. The plate was coated with different amounts of mouse IgG. A serial dilution of RM104 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. |
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